Cutting edge: recognition of Gram-positive bacterial cell wall components by the innate immune system occurs via Toll-like receptor 2.
Cutting edge: recognition of Gram-positive bacterial cell wall components by the innate immune system occurs via Toll-like receptor 2.
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DOI：
10.4049/jimmunol.163.1.1
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发表时间：
1999-07
影响因子：
4.4
通讯作者：
A. Yoshimura;E. Lien;R. Ingalls;E. Tuomanen;R. Dziarski;D. Golenbock
中科院分区：
文献类型：
--
作者：
A. Yoshimura;E. Lien;R. Ingalls;E. Tuomanen;R. Dziarski;D. Golenbock
革兰氏阳性和革兰氏阴性菌的侵袭性感染常导致感染性休克和死亡。对革兰氏阳性细菌感染的先天免疫反应的最早步骤的基础尚不清楚。革兰氏阴性细菌细胞壁的LPS成分似乎通过CD14和toll样受体（TLR） 2和TLR4激活细胞。我们假设革兰氏阳性细菌也可能被TLRs识别。NF-kappaB的诱导易位证明，在成纤维细胞中异源表达人TLR2，而不是TLR4，可使人对金黄色葡萄球菌和肺炎链球菌产生反应。CD14共表达协同增强tlr2介导的激活。为了确定革兰氏阳性细胞壁的哪些成分激活Toll蛋白，我们测试了从金黄色葡萄球菌制备的可溶性肽聚糖制剂。替代整个生物体的可溶性肽聚糖。这些数据表明，革兰氏阳性和革兰氏阴性细菌对侵袭性感染的临床反应的相似性是由于细菌通过相似的tlr识别。
Invasive infection with Gram-positive and Gram-negative bacteria often results in septic shock and death. The basis for the earliest steps in innate immune response to Gram-positive bacterial infection is poorly understood. The LPS component of the Gram-negative bacterial cell wall appears to activate cells via CD14 and Toll-like receptor (TLR) 2 and TLR4. We hypothesized that Gram-positive bacteria might also be recognized by TLRs. Heterologous expression of human TLR2, but not TLR4, in fibroblasts conferred responsiveness to Staphylococcus aureus and Streptococcus pneumoniae as evidenced by inducible translocation of NF-kappaB. CD14 coexpression synergistically enhanced TLR2-mediated activation. To determine which components of Gram-positive cell walls activate Toll proteins, we tested a soluble preparation of peptidoglycan prepared from S. aureus. Soluble peptidoglycan substituted for whole organisms. These data suggest that the similarity of clinical response to invasive infection by Gram-positive and Gram-negative bacteria is due to bacterial recognition via similar TLRs.