Drift-Tube Ion Mobility-Mass Spectrometry for Nontargeted ′Omics
Drift-Tube Ion Mobility-Mass Spectrometry for Nontargeted ′Omics
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DOI：
10.1007/978-1-0716-0030-6_4
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发表时间：
2020-01-01
期刊：
影响因子：
--
通讯作者：
Hann, Stephan
中科院分区：
文献类型：
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作者：
Causon, Tim J.;Kurulugama, Ruwan T.;Hann, Stephan
本章介绍了漂移管离子迁移质谱（DTIM-MS）的发展，推动了组学分析的应用开发。利用额外的正交分离，DTIM在化合物鉴定中提供了更高的置信度，因为质谱复杂性可以降低，并且迁移率衍生的参数（最突出的是碰撞截面，CCS）用于支持各种组学应用领域的身份确认目标。在这篇文章中提出了一种方法，用于改善传输和保持准确测定漂移时间衍生的CCS（（CCS）-C-DT）的低分子量化合物的一个典型的非靶向组学（代谢组学）的工作流程，使用液相色谱结合DTIM-MS。
This chapter describes the developments in drift-tube ion mobility-mass spectrometry (DTIM-MS) that have driven application development in 'omics analyses. Harnessing the additional, orthogonal separation that DTIM provides increased confidence in compound identifications as the mass spectral complexity can be reduced and mobility-derived parameters (most prominently the collision cross section, CCS) used to support identity confirmation goals for a variety of 'omics application areas. Presented within this contribution is a methodology for improving the transmission and maintaining accurate determination of drift time-derived CCS ((CCS)-C-DT) for low molecular weight compounds for a typical nontargeted 'omics (metabolomics) workflow using liquid chromatography in combination with DTIM-MS.