Extrinsic Sources of Cholinergic Innervation of the Striatal Complex: A Whole-Brain Mapping Analysis
Extrinsic Sources of Cholinergic Innervation of the Striatal Complex: A Whole-Brain Mapping Analysis
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DOI：
10.3389/fnana.2010.00001
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发表时间：
2016-01-22
影响因子：
2.9
通讯作者：
Mena-Seogvia, Juan
中科院分区：
文献类型：
--
作者：
Dautan, Daniel;Bay, Husniye Hacioglu;Mena-Seogvia, Juan
纹状体复合体中的乙酰胆碱在正常行为中发挥着重要作用，并在许多神经系统疾病中受到影响。尽管早期研究表明纹状体中的乙酰胆碱（SIR）几乎完全来自胆碱能中间神经元（CIN），但最近使用条件顺行追踪的轴突绘图研究揭示了从桥脚和背侧被盖核到背侧纹状体和伏隔核的显着的直接胆碱能通路的存在。识别该通路的重要性对于创建纹状体胆碱能调节的完整模型至关重要，并且它提出了其他胆碱能神经元群体是否也可能参与这种调节的问题。在这里，我们使用基于表型特异性荧光报告基因表达的新型病毒追踪技术与逆行追踪相结合，旨在确定纹状体胆碱能神经支配的其他来源。通过胆碱能轴突的条件追踪，系统地绘制大脑中所有胆碱能结构（Chi 至 Ch8）的投影，揭示胆碱能神经支配的唯一外在来源出现在脑干脚桥和外侧被盖核中。因此，我们的结果将桥脚核和外侧核置于关键且独特的位置，以提供纹状体系统活动的外在胆碱能调节。
Acetylcholine in the striatal complex plays an important role in normal behavior and is affected in a number of neurological disorders. Although early studies suggested that acetylcholine in the striatum (SIR) is derived almost exclusively from cholinergic interneurons (CIN), recent axonal mapping studies using conditional anterograde tracing have revealed the existence of a prominent direct cholinergic pathway from the pedunculopontine and laterodorsal tegmental nuclei to the dorsal striatum and nucleus accumbens. The identification of the importance of this pathway is essential for creating a complete model of cholinergic modulation in the striatum, and it opens the question as to whether other populations of cholinergic neurons may also contribute to such modulation. Here, using novel viral tracing technologies based on phenotype -specific fluorescent reporter expression in combination with retrograde tracing, we aimed to define other sources of cholinergic innervation of the striatum. Systematic mapping of the projections of all cholinergic structures in the brain (Chi to Ch8) by means of conditional tracing of cholinergic axons, revealed that the only extrinsic source of cholinergic innervation arises in the brainstem pedunculopontine and laterodorsal tegmental nuclei. Our results thus place the pedunculopontine and laterodorsal nuclei in a key and exclusive position to provide extrinsic cholinergic modulation of the activity of the striatal systems.