Effect of cytokinins on shoot regeneration from cotyledon and leaf segment of stem mustard (Brassica juncea var. tsatsai)
Effect of cytokinins on shoot regeneration from cotyledon and leaf segment of stem mustard (Brassica juncea var. tsatsai)
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DOI：
10.1007/s11240-005-3799-5
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发表时间：
2005-10-01
影响因子：
3
通讯作者：
Zheng, SJ
中科院分区：
文献类型：
--
作者：
Guo, DP;Zhu, ZJ;Zheng, SJ
茎用芥菜（Brassica juncea var. tsatsai）在添加不同浓度的细胞分裂素[6-苄基腺嘌呤（BA）、N-（2-氯-4-吡啶基）-n-苯基脲（CPPU）、6-糠基氨基嘌呤（KT）和噻苯隆（TDZ）]与不同水平的α-萘乙酸（NAA）的组合的Murashige和Skoog培养基上培养。子叶和叶段的不定芽再生频率与培养基中细胞分裂素的种类和浓度有关，但多数情况下子叶的不定芽再生频率高于叶段。TDZ是诱导子叶和叶切段不定芽的最佳细胞分裂素，BA、KT和CPPU次之。当TDZ2.27或4.54 μ M与NAA5.37 μ M组合时，子叶和叶段的不定芽再生率分别为61.3- 67.9%和40.7-52.4%。除TDZ外，CPPU也非常适合子叶和叶段不定芽的诱导。当1.61 μ M CPPU与2.69 μ M NAA组合时，子叶和叶段的不定芽再生率分别为45.0%和36.4%。KT和BA对子叶和叶段不定芽的诱导有一定的影响，NAA和细胞分裂素共同作用可显著提高不定芽的诱导率。建立了高效、可靠的子叶和叶片再生体系。该再生体系可应用于今后利用基因工程技术对该作物进行改良。
Cotyledon and leaf segments of stem mustard (Brassica juncea var. tsatsai) were cultured on Murashige and Skoog medium supplemented with various concentrations of different cytokinins [6-benzyladenine (BA), N-(2-chloro-4-pyridyl)-n-phenylurea (CPPU), 6-furfurylaminopurine (KT) and thidiazuron (TDZ)] in combinations with different levels of alpha-naphthalene acetic acid (NAA). The shoot regeneration frequency of cotyledon and leaf segment was dependent on the kinds and concentrations of cytokinins used in the medium, while in most cases cotyledon gave high regeneration frequency than leaf segment. TDZ proved to be the best cytokinin to induce shoot from both cotyledon and leaf segments compared to BA, KT and CPPU. The highest frequency of shoot regeneration was 61.3-67.9 % in cotyledon and 40.7-52.4% in leaf segment respectively when 2.27 or 4.54 mu M TDZ was combined with 5.37 mu M NAA. Next to TDZ, CPPU was also very suitable to induce shoot formation both in cotyledon and leaf segment. When 1.61 mu M CPPU was combined with 2.69 mu M NAA, shoot regeneration frequency was 45.0% in cotyledon and 36.4% in leaf segment, respectively. It was also shown that KT and BA affected shoot regeneration from cotyledon and leaf segment, the shoot regeneration was greatly increased when NAA was added together with cytokinins. The efficient and reliable shoot regeneration system was developed in both cotyledon and leaf segments. This regeneration protocol may be applicable to the improvement of this crop by genetic engineering in the future.