Cloning of a high-affinity K+ transporter gene PutHKT2;1 from Puccinellia tenuiflora and its functional comparison with OsHKT2;1 from rice in yeast and Arabidopsis.
Cloning of a high-affinity K+ transporter gene PutHKT2;1 from Puccinellia tenuiflora and its functional comparison with OsHKT2;1 from rice in yeast and Arabidopsis.
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DOI：
10.1093/jxb/erp184
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发表时间：
2009
影响因子：
6.9
通讯作者：
Takano T
中科院分区：
文献类型：
--
作者：
Ardie SW;Xie L;Takahashi R;Liu S;Takano T
从耐盐植物星星草（Puccinellia tenuiflora）中克隆了一个高亲和力K+转运蛋白PutHKT 2;1的cDNA。PutHKT 2;1的表达诱导300 mM NaCl和K+饥饿胁迫在根中，但只有轻微的调节，这些应力在地上部。PutHKT 2;1在300 mM NaCl中的转录水平通过钾的耗尽而加倍。用PutHKT 2;1转化的酵母，与用来自耐盐芦苇（Phragmites australis）的PhaHKT 2;1转化的酵母一样，（i）能够在低K+浓度培养基中或在NaCl存在下吸收K+，并且（ii）对Na+是可渗透的。这表明PutHKT 2;1在酵母中具有高亲和力的K+-Na+同向转运功能。过表达PutHKT 2;1的拟南芥对Na+、K+和Li+的敏感性增加，而过表达水稻OsHKT 2;1的拟南芥仅对Na+的敏感性增加。与OsHKT 2相比; PutHKT 2;1在高K+浓度下起Na+吸收作用。这些结果表明PutHKT 2;1在转基因酵母和拟南芥中的作用模式不同于密切相关的OsHKT 2;1转运蛋白的作用模式。
A high-affinity K+ transporter PutHKT2;1 cDNA was isolated from the salt-tolerant plant Puccinellia tenuiflora. Expression of PutHKT2;1 was induced by both 300 mM NaCl and K+-starvation stress in roots, but only slightly regulated by those stresses in shoots. PutHKT2;1 transcript levels in 300 mM NaCl were doubled by the depletion of potassium. Yeast transformed with PutHKT2;1, like those transformed with PhaHKT2;1 from salt-tolerant reed plants (Phragmites australis), (i) were able to take up K+ in low K+ concentration medium or in the presence of NaCl, and (ii) were permeable to Na+. This suggests that PutHKT2;1 has a high affinity K+-Na+ symport function in yeast. Arabidopsis over-expressing PutHKT2;1 showed increased sensitivities to Na+, K+, and Li+, while Arabidopsis over-expressing OsHKT2;1 from rice (Oryza sativa) showed increased sensitivity only to Na+. In contrast to OsHKT2;1, which functions in Na+-uptake at low external K+ concentrations, PutHKT2;1 functions in Na+-uptake at higher external K+ concentrations. These results show that the modes of action of PutHKT2;1 in transgenic yeast and Arabidopsis differ from the mode of action of the closely related OsHKT2;1 transporter.
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DOI：
10.1073/pnas.082123799
发表时间：
2002-04-30
影响因子：
11.1
作者：
Mäser, P;Hosoo, Y;Uozumi, N
通讯作者：
Uozumi, N
影响因子：
4.9
作者：
Horie, Tomoaki;Horie, Rie;Schroeder, Julian I.
通讯作者：
Schroeder, Julian I.
影响因子：
7.4
作者：
Liu, WH;Fairbairn, DJ;Schachtman, DP
通讯作者：
Schachtman, DP
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
影响因子：
7.2
作者：
Gassmann, W;Rubio, F;Schroeder, JI
通讯作者：
Schroeder, JI