Kanamycin detection based on the catalytic ability enhancement of gold nanoparticles
Kanamycin detection based on the catalytic ability enhancement of gold nanoparticles
复制标题
基于金纳米颗粒催化能力增强的卡那霉素检测
DOI：
10.1016/j.bios.2016.12.042
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发表时间：
2017-05-15
影响因子：
12.6
通讯作者：
Tan, Rong
中科院分区：
文献类型：
--
作者：
Wang, Chengke;Chen, Dan;Tan, Rong
在本文中，我们证明了卡那霉素可以通过两个步骤增强柠檬酸盐修饰的金纳米颗粒（AuNPs）的过氧化物酶样活性：通过-NH 2将卡那霉素连接到AuNPs上（卡那霉素）和-COOH（在AuNPs上）相互作用;以及卡那霉素上的糖苷与金纳米粒子的特异性相互作用，改变了金纳米粒子的表面性质，在溶液中产生了OH自由基和Au ~（3+），催化3，3 ′，5，5 ′-四甲基联苯胺（TMB）与H_2O_2的显色反应。基于这一原理，建立了一种检测卡那霉素的新方法。该方法具有较高的灵敏度和选择性，可检测低至0.1 nM的卡那霉素，线性范围分别为0.1 nM至20 nM和20 nM至300 nM。该方法也成功地应用于牛奶和肉类样品中卡那霉素含量的检测。
In this paper, we demonstrated that kanamycin could enhance the peroxidase-like activity of citrate-capped gold nanoparticles (AuNPs) through two steps: the attachment of kanamycin onto AuNPs through -NH2 (on kanamycin) and -COOH (on AuNPs) interactions; and the specifically interaction between glucoside on kanamycin and AuNPs which changes the surface property of AuNPs, and produced "OH radicals and Au3+ in the solution, and catalyzed the chromogenic reactions between 3, 3', 5, 5'-tetramethylbenzidine (TMB) and H2O2. Based on this principle, a novel method for kanamycin detection has been developed. This method exhibited high sensitivity and selectivity, as low as 0.1 nM kanamycin could be detected with a linear range from 0.1 nM to 20 nM and 20nM to 300nM, respectively. This method was also successfully applied for the detection of kanamycin content in milk and meat samples.