LcEIL2/3 are involved in fruitlet abscission via activating genes related to ethylene biosynthesis and cell wall remodeling in litchi
LcEIL2/3 are involved in fruitlet abscission via activating genes related to ethylene biosynthesis and cell wall remodeling in litchi
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LcEIL2/3 通过激活与荔枝乙烯生物合成和细胞壁重塑相关的基因参与果实脱落
DOI：
10.1111/tpj.14804
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发表时间：
2020-06-10
期刊：
影响因子：
7.2
通讯作者：
Zhao, Minglei
中科院分区：
文献类型：
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作者：
Ma, Xingshuai;Yuan, Ye;Zhao, Minglei
水果作物容易出现早熟果实脱落，在此期间植物激素乙烯（ET）充当主要的正调节剂。然而，ET 诱导的果实脱落的分子基础仍然知之甚少。在这里，我们发现荔枝中的两个乙烯不敏感3样（EIL）同源物LcEIL2和LcEIL3在ET激活的果实脱落中发挥作用。在ET诱导的荔枝果实脱落过程中，LcEIL2/3在果实脱落区（AZ）显着上调。野生型拟南芥andein3 eil1突变体中LcEIL2/3的存在可以加速花器官的脱落。此外，电泳迁移率变动实验和双荧光素酶报告基因分析表明，LcEIL2/3直接与基因启动子相互作用，激活细胞壁重塑基因LcCEL2/8和LcPG1/2以及ET生物合成基因LcACS1/4/7和LcACO2/3的表达。此外，我们还发现LcPG1/2在拟南芥的花器官脱落区表达，并且LcPG2在拟南芥中的组成型表达促进了花器官的脱落。总之，我们认为 LcEIL2/3 通过控制荔枝中 ET 生物合成和细胞壁重塑相关基因的表达参与 ET 诱导的果实脱落。
Fruit crops are subject to precocious fruit abscission, during which the phytohormone ethylene (ET) acts as a major positive regulator. However, the molecular basis of ET-induced fruit abscission remains poorly understood. Here, we show that two ETHYLENE INSENSITIVE 3-like (EIL) homologs in litchi,LcEIL2andLcEIL3, play a role in ET-activated fruitlet abscission.LcEIL2/3were significantly upregulated in the fruit abscission zone (AZ) during the ET-induced fruitlet abscission in litchi. The presence ofLcEIL2/3in wild-type Arabidopsis andein3 eil1mutants can accelerate the floral organ abscission. Moreover, the electrophoretic mobility shift assay and dual luciferase reporter analysis illustrated that LcEIL2/3 directly interacted with the gene promoters to activate the expression of cell wall remodeling genesLcCEL2/8andLcPG1/2, and ET biosynthetic genesLcACS1/4/7andLcACO2/3. Furthermore, we showed thatLcPG1/2were expressed in the floral abscission zone of Arabidopsis, and constitutive expression ofLcPG2in Arabidopsis promoted the floral organ abscission. In conclusion, we propose that LcEIL2/3 are involved in ET-induced fruitlet abscission via controlling expression of genes related to ET biosynthesis and cell wall remodeling in litchi.