Elevated Cytokine Levels in the Aqueous Humor of Eyes With Bullous Keratopathy and Low Endothelial Cell Density
Elevated Cytokine Levels in the Aqueous Humor of Eyes With Bullous Keratopathy and Low Endothelial Cell Density
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DOI：
10.1167/iovs.16-20187
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发表时间：
2016-11-01
影响因子：
4.4
通讯作者：
Shimazaki, Jun
中科院分区：
文献类型：
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作者：
Yamaguchi, Takefumi;Higa, Kazunari;Shimazaki, Jun
目的.目的探讨大泡性角膜病变（BK）合并低内皮细胞密度（ECD）患者眼房水（AqH）中细胞因子水平的变化。从接受角膜移植或白内障手术的连续患者中共收集了145个AqH样本（60个BK，16个低ECD，35个具有正常ECD的角膜疾病和34个正常对照）。在AqH收集时，没有患者具有任何临床上明显的炎症。细胞因子AqH水平（IL-1 α、IL-1 β、IL-2 β、IL-4、IL-6、IL-8、IL-10、IL-12 p70、IL-13、IL-17 A、IFN-α、IFN-γ、单核细胞趋化蛋白[MCP]1、TNF-α、E-选择素、P-选择素、可溶性细胞间粘附分子[sICAM]-1、粒细胞-巨噬细胞集落刺激因子[GM-CSF]、巨噬细胞炎性蛋白[MIP]-1 α和MIP-1 β）。BK和低ECD眼AqH中IL-1 α、IL-8、IL-17 A、TNF-α、GM-CSF、MIP-1 α、IFN-γ和E-选择素的水平与健康对照组相比显著升高（均P < 0.03）。BK组IL-4、IL-6、IL-10、IL-12 p70、IL-13、MCP-1、MIP-1 β、P-选择素和sICAM-1水平均显著高于正常对照组（P均< 0.001）。ECD正常的角膜疾病与正常对照眼之间AqH细胞因子水平无显著差异。在BK眼中，IL-6和IFN-γ水平在人工晶状体BK（PBK）、Fuchs角膜内皮营养不良（FECD）、角膜移植术后、小梁切除术后和激光虹膜切开术（LI）后升高。（所有，P < 0.04），而IL-1 α、IL-10、IL-17 A、MIP-1 β和sICAM-1水平仅在PBK、角膜移植术后、小梁切除术后、LI术后眼与术后眼比较，差异有统计学意义（P < 0.05）。AqH细胞因子水平的亚临床升高可能导致内皮细胞损失。
PURPOSE. To evaluate cytokine levels in the aqueous humor (AqH) of eyes with bullous keratopathy (BK) and low endothelial cell density (ECD).METHODS. A total of 145 AqH samples (60 BK, 16 low ECD, 35 corneal diseases with normal ECD, and 34 normal controls) were collected from consecutive patients who underwent corneal transplantation or cataract surgery. None of the patients had any clinically apparent inflammation at the time of AqH collection. The AqH levels of cytokines (IL-1 alpha, IL-1 beta, IL-2 beta, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-17A, IFN-alpha, IFN-gamma, monocyte chemotactic protein [MCP]1, TNF-alpha, E-selectin, P-selectin, soluble intercellular adhesion molecule [sICAM]-1, granulocyte-macrophage colony-stimulating factor [GM-CSF], macrophage inflammatory protein [MIP]-1 alpha and MIP-1 beta) were compared among the groups.RESULTS. The levels of IL-1 alpha, IL-8, IL-17A, TNF-alpha, GM-CSF, MIP-1 alpha, IFN-gamma, and E-selectin in the AqH were significantly elevated in BK and low ECD eyes, compared with healthy controls (all P < 0.03). The levels of IL-4, IL-6, IL-10, IL-12p70, IL-13, MCP-1, MIP-1 beta, P-selectin, and sICAM-1 were significantly elevated only in BK eyes, compared with healthy control (all P < 0.001). There were no significant differences in AqH cytokine levels between corneal diseases with normal ECD and normal control eyes. Among BK eyes, the IL-6 and IFN-gamma levels were elevated in eyes with pseudophakic BK (PBK), Fuchs' endothelial corneal dystrophy (FECD), postkeratoplasty, posttrabeculectomy, and postlaser iridotomy (LI) (all, P < 0.04), whereas IL-1 alpha, IL-10, IL-17A, MIP-1 beta, and sICAM-1 levels were elevated only in PBK, postkeratoplasty, posttrabeculectomy, and post-LI eyes (all, P < 0.05).CONCLUSIONS. Subclinical elevation of AqH cytokine levels may cause endothelial cell loss.