Static and time-resolved fluorescence investigations of tryptophan analogues - a solvent study
Static and time-resolved fluorescence investigations of tryptophan analogues - a solvent study
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DOI：
10.1039/b312436c
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发表时间：
2004-01-01
影响因子：
3.1
通讯作者：
Brockhinke, A
中科院分区：
文献类型：
--
作者：
Lotte, K;Plessow, R;Brockhinke, A
色氨酸，聚色氨酸及其类似物（7-氮杂色氨酸，5-羟基色氨酸，5-甲氧基色氨酸，5-氟色氨酸，5-甲基色氨酸，5-溴色氨酸，和6-氟色氨酸）的荧光性质进行了研究，使用两种新的荧光光谱技术，在很宽的溶剂极性范围内。使用激发-发射光谱（EES）对所有发射光谱和所有荧光光谱进行二维映射已用于确定量子产率、发射最大值位置、半峰全宽（FWHM）以及斯托克斯位移。此外，荧光寿命从时间分辨的实验中使用皮秒激光系统，并从静态设置获得的数据进行比较。这种系统的荧光特性的研究是一个先决条件，以评估这些类似物的潜力，作为结构保守的替代色氨酸在蛋白质荧光实验。这些类似物的潜力，作为当地环境的探针，并允许估计的极性在附近的荧光团和其暴露于溶剂，进行了讨论。
The fluorescence properties of tryptophan, polytryptophan and seven of its analogues (7-azatryptophan, 5-hydroxytryptophan, 5-methoxytryptophan, 5-fluorotryptophan, 5-methyltryptophan, 5-bromotryptophan, and 6-fluorotryptophan) are studied using two novel fluorescence spectroscopic techniques for a wide range of solvent polarities. Two-dimensional mapping of all emission and all fluorescence spectra using excitation-emission spectroscopy (EES) has been used to determine quantum yields, positions of emission maxima, full widths at half maximum (FWHMs) as well as Stokes' shifts. Additionally, fluorescence lifetimes obtained from time-resolved experiments using a picosecond laser system are presented and compared with the data acquired from the static setup. This systematic study of the fluorescence characteristics is a prerequisite to assess the potential of these analogues to act as structure-conserving substitutes for tryptophan in protein fluorescence experiments. The potential of these analogues, to act as probes for the local environment, and allow estimation of the polarity in the vicinity of the fluorophore and its exposure to the solvent, is discussed.