Effect of opioid receptor antagonists on vasodilator nerve actions in the perfused rat mesentery.
Effect of opioid receptor antagonists on vasodilator nerve actions in the perfused rat mesentery.
复制标题
阿片受体拮抗剂对灌注大鼠肠系膜血管舒张神经作用的影响。
DOI：
10.1016/0014-2999(91)90859-o
复制
发表时间：
1991
影响因子：
5
通讯作者：
Duckles,SP
中科院分区：
文献类型：
--
作者：
Li,YJ;Duckles,SP
我们之前的工作表明阿片肽调节灌注大鼠肠系膜的感觉神经。因此，我们通过选择性阿片受体拮抗剂测试了阿片样物质参与感觉神经活动持续调节的假设。在冠脉定和甲氧沙明存在的情况下，经壁神经刺激引起血管舒张反应，纳洛酮（3 × 10−3M）显著增强了血管舒张反应。然而，纳洛酮不影响血管舒张剂对外源性降钙素基因相关肽的反应。ICI 174.864 （3 × 10−7M）是一种选择性δ受体拮抗剂，对血管扩张剂对跨壁神经刺激的反应没有影响。相比之下，选择性μ受体拮抗剂CTOP (d - ph - cys - tyr - d - trp - orn - thr - phc - thr - nh2) （3 × 10−7M）显著抑制血管舒张剂对跨壁神经刺激的反应，这一作用被纳洛酮治疗所消除。在用β-FNA （β-FNA检测HCI）的制剂中。一个不可逆的μ。受体拮抗剂，纳洛酮不再增强血管扩张剂对跨壁神经刺激的反应。这些结果表明，纳洛酮对跨壁神经刺激的血管扩张反应增强可能是由于μ受体的阻断，导致内源性阿片的抑制调节减弱。这些发现支持了感觉神经上的前置阿片受体可能在调节心血管系统活动中发挥作用的论点。
Our previous work suggests that opioid peptidcs modulate sensory nerves in the perfused rat mesentery. Therefore we tested the hypothesis that opioids are involved in the ongoing regulation of sensory nerve activity using selective opioid receptor antagonists. In the presence of guancthidinc and methoxamine, transmural nerve stimulation caused a vasodilator response which was potentiated significantly by naloxonc (3 × 10−3M). However, naloxone did not affect vasodilator responses to exogenous calcitonin gene related peptide. ICI 174.864 (3 × 10−7M), a selective δ receptor antagonist, had no effect on vasodilator responses to transmural nerve stimulation. In contrast CTOP (D-Phe-Cys-Tyr-D-Trp-Orn-Thr-Phc-Thr-NH2) (3 × 10−7M), a selective μ receptor antagonist, significantly inhibited vasodilator responses to transmural nerve stimulation, effects which were abolished by naloxone treatment. In preparations prctrcalcd with β-FNA (β-funaltrexaminc HCI). an irreversible μ. receptor antagonist, naloxone no longer potentiated vasodilator responses to transmural nerve stimulation. These results suggest that potentiation of vasodilator responses to transmural nerve stimulation by naloxone may be due to blockade of μ receptors, resulting in a reduced inhibitory modulation by endogenous opioids. These findings support the contention that prejunctionai opioid receptors on sensory nerves may play a role in moduluting activity of the cardiovascular system.