Pulsed-field gel electrophoresis typing of oxacillin-resistant Staphylococcus aureus isolates from the United States:: Establishing a national database
Pulsed-field gel electrophoresis typing of oxacillin-resistant Staphylococcus aureus isolates from the United States:: Establishing a national database
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DOI：
10.1128/jcm.41.11.5113-5120.2003
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发表时间：
2003-11-01
影响因子：
9.4
通讯作者：
Tenover, FC
中科院分区：
文献类型：
--
作者：
McDougal, LK;Steward, CD;Tenover, FC
苯唑西林耐药金黄色葡萄球菌（ORSA）是一种致病性病原体，可引起卫生保健相关疾病和社区发病疾病。我们使用脉冲场凝胶电泳（PFGE）分离的SmaI消化的基因组DNA来表征957 S。并建立PFGE图谱数据库。除了美国菌株的PFGE模式外，该数据库还包含来自英国、加拿大和澳大利亚的代表性肺炎克雷伯菌型菌株的模式;先前描述的ORSA克隆型分离株; 13种万古霉素中间体S.金黄色葡萄球菌（VISA）分离株，和两个高水平万古霉素耐药，vanA阳性菌株（VRSA）。在来自美国的分离株中，我们鉴定了8个谱系，命名为脉冲场型（PFT）USA 100至USA 800，其中7个既包括ORSA又包括苯唑西林敏感的S。金黄色葡萄球菌分离株。除了PFT对USA 100和USA 800以及USA 300和USA 500之外，每个PFT都具有独特的多位点序列类型和spa型基序。USA 100 PFT，以前被指定为纽约/东京克隆，是数据库中最常见的PFT，代表44 10个ORSA分离株。USA 100分离株通常具有多重耐药性，包括除一种美国VISA菌株外的所有菌株和两种VRSA分离株。来自USA 200、-500和600 PFT的多重耐药ORSA分离株具有与先前描述的来自欧洲和澳大利亚的流行株相似的PFGE模式。USA 300和-400 PFT包含仅对β-内酰胺类药物和红霉素耐药的社区分离株。值得注意的是，美国数据库中未发现具有先前描述的巴西和EMRSA 15 PFGE模式的分离株。这些数据表明，在美国存在有限数量的ORSA基因型。
Oxacillin-resistant Staphylococcus aureus (ORSA) is a virulent pathogen responsible for both health care-associated and community onset disease. We used SmaI-digested genomic DNA separated by pulsed-field gel electrophoresis (PFGE) to characterize 957 S. aureus isolates and establish a database of PFGE patterns. In addition to PFGE patterns of U.S. strains, the database contains patterns of representative epidemic-type strains from the United Kingdom, Canada, and Australia; previously described ORSA clonal-type isolates; 13 vancomycin-intermediate S. aureus (VISA) isolates, and two high-level vancomycin-resistant, vanA-positive strains (VRSA). Among the isolates from the United States, we identified eight lineages, designated as pulsed-field types (PFTs) USA100 through USA800, seven of which included both ORSA and oxacillin-susceptible S. aureus isolates. With the exception of the PFT pairs USA100 and USA800, and USA300 and USA500, each of the PFTs had a unique multilocus sequence type and spa type motif. The USA100 PFT, previously designated as the New York/Tokyo clone, was the most common PFT in the database, representing 44 10 of the ORSA isolates. USA100 isolates were typically multiresistant and included all but one of the U.S. VISA strains and both VRSA isolates. Multiresistant ORSA isolates from the USA200, -500, and -600 PFTs have PFGE patterns similar to those of previously described epidemic strains from Europe and Australia. The USA300 and -400 PFTs contained community isolates resistant only to beta-lactam drugs and erythromycin. Noticeably absent from the U.S. database were isolates with the previously described Brazilian and EMRSA15 PFGE patterns. These data suggest that there are a limited number of ORSA genotypes present in the United States.