MOVAS-1 cell line: A new in vitro model of vascular calcification
MOVAS-1 cell line: A new in vitro model of vascular calcification
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DOI：
10.3892/ijmm.2011.631
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发表时间：
2011-05-01
影响因子：
5.4
通讯作者：
MacRae, V. E.
中科院分区：
文献类型：
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作者：
Mackenzie, N. C. W.;Zhu, D.;MacRae, V. E.
血管钙化在包括糖尿病、动脉粥样硬化和终末期肾病在内的许多疾病中具有严重的临床后果。体外培养的小鼠、人和牛血管平滑肌细胞（VSMCs）的钙化常被用于研究血管钙化的机制。然而，到目前为止，还没有发表的研究利用鼠细胞系来研究该过程。在本研究中，我们的目的是确定小鼠VSMC系MOVAS-1是否可以在体外钙化。我们建立了在钙化培养基（含有β-甘油磷酸盐和抗坏血酸）的存在下可以诱导MOVAS-1细胞的钙化，如通过茜素红和von Kossa染色以及钙沉积和碱性磷酸酶活性的定量所检测的。我们还表明，MOVAS-1钙化的时程与原代鼠主动脉VSMC的时程相当，从而将MOVAS-1细胞确立为可行且相关的模型。在钙化条件下培养的MOVAS-1细胞中观察到与血管钙化相关的关键基因（Ocn、Akp 2和PiT-1）的mRNA表达谱的显著增加，在小鼠主动脉VSMC中的表达具有类似的变化。此外，在用已知的钙化抑制剂左旋咪唑和依替膦酸盐处理后，在MOVAS-1细胞中观察到钙化的显著减少。总之，我们证明了MOVAS-1线是一种可靠、方便和经济的系统，可用于研究体外血管钙化，并将为增加我们对这一病理过程的理解做出有益的贡献。
Vascular calcification has severe clinical consequences in a number of diseases, including diabetes, atherosclerosis and end-stage renal disease. The in vitro calcification of primary mouse, human and bovine vascular smooth muscle cells (VSMCs) is commonly employed to examine the mechanisms of vascular calcification. However, to date, no published studies have utilised a murine cell line to investigate this process. In the present study, we aimed to determine whether the mouse VSMC line MOVAS-1 can calcify in vitro. We established that the calcification of MOVAS-1 cells can be induced in the presence of calcifying medium (containing beta-glycerophosphate and ascorbic acid), as detected by Alizarin Red and von Kossa staining, and quantification of calcium deposition and alkaline phosphatase activity. We also showed that the time course of MOVAS-1 calcification is comparable to that of the primary murine aortic VSMCs, establishing the MOVAS-1 cells as a feasible and relevant model. Significant increases in the mRNA expression profile of key genes associated with vascular calcification (Ocn, Akp2 and PiT-1) were observed in MOVAS-1 cells cultured under calcifying conditions, with similar changes in expression in murine aortic VSMCs. Furthermore, a significant reduction in calcification was observed in MOVAS-1 cells following treatment with levamisole and etidronate, known inhibitors of calcification. In conclusion, we demonstrated that the MOVAS-1 line is a reliable, convenient and economical system in which to investigate, vascular calcification in vitro, and will make a useful contribution to increasing our understanding of this pathological process.