Dynamin II is involved in endocytosis but not in the formation of transport vesicles from the trans-Golgi network
Dynamin II is involved in endocytosis but not in the formation of transport vesicles from the trans-Golgi network
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DOI：
10.1093/oxfordjournals.jbchem.a022349
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发表时间：
1999-04-01
影响因子：
2.7
通讯作者：
Nakayama, K
中科院分区：
文献类型：
--
作者：
Kasai, K;Shin, HW;Nakayama, K
发动蛋白是一个类似于100-kDa GTP酶的家族，被认为在内吞包被囊泡的形成中起关键作用。在哺乳动物中有三种动力蛋白基因：动力蛋白I是神经元特异性的，动力蛋白II显示普遍表达，而动力蛋白III在睾丸、脑和肺中表达。然而，迄今为止，大多数关于发动蛋白功能的研究仅限于发动蛋白I。在本研究中，我们表明，与发动蛋白I一样，发动蛋白II参与受体介导的内吞作用。豪厄尔，K.E.，亨利，J.R.，曹，H.，和McNiven，M.A.（1998）Science 279，573-577]报道了发动蛋白II定位于高尔基体网络（trans-Golgi network，TGN）中，并参与从该隔室形成组成型运输囊泡和网格蛋白包被的囊泡。然而，免疫荧光分析和实验中使用的细胞转染显性阴性发动蛋白II未能显示任何证据的本地化的发动蛋白II在TGN或其参与囊泡形成从这个隔间，因此，我们的数据表明，发动蛋白II参与内吞作用，但不是在形成运输囊泡从TGN。
Dynamins are a family of similar to 100-kDa GTPases that are thought to play a pivotal role in the formation of endocytic coated vesicles. There are three dynamin genes in mammals: dynamin I is neuron specific, dynamin II shows ubiquitous expression, and dynamin III is expressed in testis, brain, and lung. However; most studies on the functions of dynamins to date have been restricted to dynamin I. In the present study, we show that, like dynamin I, dynamin II is involved in receptor-mediated endocytosis, While this study was in progress, Jones et al, [Jones, S.M., Howell, K.E., Henley, J.R., Cao, H., and McNiven, M.A. (1998) Science 279, 573-577] reported that dynamin II is localized in the trans-Golgi network (TGN) and involved in the formation of constitutive transport vesicles and clathrin-coated vesicles from this compartment. However, immunofluorescence analyses and experiments using cells transfected with dominant-negative dynamin II failed to show any evidence for localization of dynamin II in the TGN or for its involvement in vesicle formation from this compartment, Our data thus indicate that dynamin II is involved in endocytosis but not in the formation of transport vesicles from the TGN.