Secondary structure in formylmethionine tRNA influences the site-directed cleavage of ribonuclease H using chimeric 2'-O-methyl oligodeoxyribonucleotides.
Secondary structure in formylmethionine tRNA influences the site-directed cleavage of ribonuclease H using chimeric 2'-O-methyl oligodeoxyribonucleotides.
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甲酰甲硫氨酸 tRNA 中的二级结构影响使用嵌合 2-O-甲基寡脱氧核糖核苷酸对核糖核酸酶 H 进行定点切割。
作者：
Y. Hayase;H. Inoue;E. Ohtsuka
为了在大肠杆菌甲硫氨酸tRNA、RNaseH和由DNA和2‘-O-甲基-RNA组成的互补嵌合寡核苷酸中切割特定位置的RNA(Inoue等人)。(1987)FEBS Lett.215,327]。研究了D环、反密码子环、T psi C环、反密码子茎和受体茎中的特异性切割。当使用相对较长的嵌合寡核苷酸(20-mer)时，在T psi C环、反密码子茎和受体茎上观察到几乎唯一的RNaseH水解酶。当tRNA的高级结构通过与受体的20-聚体和T-psi C茎区域杂交而被打破时，嵌合的13-聚体在反密码子上获得了有效的切割。结果发现，嵌合寡核苷酸杂交体的稳定性和少量核苷的存在影响了这种方法对tRNA的切割。
In order to cleave RNA at specific positions in Escherichia coli formylmethionine tRNA, RNase H and complementary chimeric oligonucleotides consisting of DNA and 2'-O-methyl-RNA (Inoue et al. (1987) FEBS Lett. 215, 327] were used. Specific cleavages in the D loop, anticodon loop, T psi C loop, anticodon stem, and acceptor stem were investigated. Virtually unique hydrolyses with RNase H were observed at the T psi C loop, anticodon stem, and acceptor stem when relatively longer chimeric oligonucleotides (20-mer) were used. An efficient cleavage at the anticodon was obtained with a chimeric 13-mer when the higher structure of the tRNA was broken by hybridization with a 20-mer at the acceptor as well as the T psi C stem region. It was found that stabilities of hybrids with chimeric oligonucleotides and the presence of minor nucleosides affect the cleavage of tRNA by this approach.
甲硫氨酰-tRNA 合成酶识别甲酰甲硫氨酸 tRNA 的反密码子环大小和序列要求。
DOI：
10.1073/pnas.80.22.6755
发表时间：
1983
影响因子：
11.1
作者：
Schulman,LH;Pelka,H
通讯作者：
Pelka,H
反密码子摆动位置的碱基取代抑制大肠杆菌 Met-tRNA 合成酶对大肠杆菌 tRNAfMet 的氨酰化。
影响因子：
14.9
作者：
Schulman,LH;Pelka,H;Susani,M
通讯作者：
Susani,M