[The identification of Cryptosporidium species (protozoa) in Ifsahan, Iran by PCR-RFLP analysis of the 18S rRNA gene].
[The identification of Cryptosporidium species (protozoa) in Ifsahan, Iran by PCR-RFLP analysis of the 18S rRNA gene].
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发表时间：
2007
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通讯作者：
M. Azami;D. D. Moghaddam-D.;R. Salehi;M. Salehi
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作者：
M. Azami;D. D. Moghaddam-D.;R. Salehi;M. Salehi
隐孢子虫是一种重要的原生动物，可引起人类和动物腹泻疾病。已报道了不同种类的隐孢子虫，据信物种特征是控制战略规划中需要考虑的重要因素。因此，我们通过 PCR-RFLP 分析了人类和动物隐孢子虫分离株的卵囊，以确定伊斯法罕的菌株变异。总共检查了来自伊斯法罕五岁以下儿童、免疫功能低下患者和高危人群的 642 份人类粪便样本以及 480 份随机选择的奶牛和小牛的直肠样本。显微镜检查显示，4.7%（30/642）的人类样本和6.2%（30/480）的动物样本感染隐孢子虫。鉴定出感染寄生虫的样本后，纯化卵囊并提取其 DNA。我们对 18S rRNA 基因的 1750 bp 区域进行 PCR-RFLP 分析来鉴定隐孢子虫属物种。人类样本感染了隐孢子虫 II、C. muris、C. wrairi 和新基因型的隐孢子虫（GenBank 登录号：DQ520951）。牛样本被鉴定为 C. parvum II、C. muris、C. wrairi、C. serpentis、C. baileyi 和隐孢子虫的新基因型（GenBank 登录号：DQ520952）。我们还发现了一种感染人类和牛样本的新基因型（GenBank 登录号：DQ520950）。除了证明大多数隐孢子虫、微小隐孢子虫物种的广泛存在之外，我们还观察到物种内广泛的多态性。此外，动物和人类样本中出现相同种类的寄生虫表明了动物-人类循环的重要性。
Cryptosporidium is an important protozoan that cause diarrheal illness in humans and animals. Different species of Cryptosporidium have been reported and it is believed that species characteristics are an important factor to be considered in strategic planning for control. We therefore analyzed oocysts from human and animal isolates of Cryptosporidium by PCR-RFLP to determine strain variation in Isfahan. In total, 642 human fecal samples from children under five years of age, immunocompromised patients, and high risk persons and 480 randomly selected rectal specimens of cows and calves in Isfahan were examined. Microscopic examination showed that 4.7% (30/642) of human samples and 6.2% (30/480) of animal samples were infected with Cryptosporidium. After identification of the samples infected with the parasite, oocysts were purified and their DNA was extracted. We used PCR-RFLP analysis of a 1750-bp region of 18S rRNA gene to identify Cryptosporidium species. The human samples were infected with Cryptosporidium parvum II, C. muris, C. wrairi, and a new genotype of Cryptosporidium (GenBank accession numbers: DQ520951). The cattle samples were identified as C. parvum II, C. muris, C. wrairi, C. serpentis, C. baileyi, and a new genotype of Cryptosporidium (GenBank accession numbers: DQ520952). Also we found a new genotype infecting both human and cattle samples (GenBank accession numbers: DQ520950). In addition to demonstrating the widespread occurrence of most species of Cryptosporidium, C. parvum, we also observed extensive polymorphism within species. Furthermore, the occurrence of the same species of parasite in both animal and human samples shows the importance of the animal-human cycle.