Activation of the cytoplasmic c-Abl tyrosine kinase by reactive oxygen species
Activation of the cytoplasmic c-Abl tyrosine kinase by reactive oxygen species
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DOI：
10.1074/jbc.c000099200
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发表时间：
2000-06-09
影响因子：
4.8
通讯作者：
Kufe, D
中科院分区：
文献类型：
--
作者：
Sun, XG;Majumder, P;Kufe, D
广泛表达的c-Abl蛋白酪氨酸激酶定位于细胞核和细胞质。c-Abl的核形式在对遗传毒性应激的细胞应答中被激活。在这里，我们表明，细胞质c-Abl被激活的氧化应激，结果还表明，线粒体细胞色素c释放的细胞响应H2 O2，这种效果是由c-Abl依赖的机制介导的。与这些结果相一致，我们表明，H2 O2诱导的细胞凋亡在c-Abl缺陷细胞中减弱。这些发现表明，细胞质c-Abl参与细胞对氧化应激的凋亡反应。
The ubiquitously expressed c-Abl protein tyrosine kinase localizes to both the nucleus and cytoplasm. The nuclear form of c-Abl is activated in the cellular response to genotoxic stress. Here we show that cytoplasmic c-Abl is activated by oxidative stress, The results also demonstrate that mitochondrial cytochrome c is released in the cellular response to H2O2 and that this effect is mediated by a c-Abl-dependent mechanism. In concert with these results, we show that H2O2-induced apoptosis is attenuated in c-Abl-deficient cells. These findings demonstrate that cytoplasmic c-Abl is involved in the apoptotic response of cells to oxidative stress.