Alteration of microRNA profiles in squamous cell carcinoma of the head and neck cell lines by human papillomavirus.
Alteration of microRNA profiles in squamous cell carcinoma of the head and neck cell lines by human papillomavirus.
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DOI：
10.1002/hed.21475
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发表时间：
2011-04
影响因子：
2.9
通讯作者：
Khan, Saleem A.
中科院分区：
文献类型：
--
作者：
Wald, Abigail I.;Hoskins, Elizabeth E.;Wells, Susanne I.;Ferris, Robert L.;Khan, Saleem A.
人乳头瘤病毒(HPV)阳性的头颈部鳞状细胞癌(SCCHN)患者与HPV阴性的SCCHN患者相比，其疾病预后要好得多。MicroRNA(MiRNA)表达的差异可能会影响它们的临床结果。应用基因芯片和定量RT-PCR技术检测HPV-16阳性和HPV阴性SCCHN细胞中miRNA的表达。利用人包皮角质形成细胞(HFK)研究了HPV-16E6和E7癌基因在改变miRNA表达中的作用。HPV阳性细胞中miR-363、miR-33和miR-497表达上调，miR-155、miR-181a、miR-181b、miR-29a、miR-218、miR-222、miR-221和miR-142-5p表达下调。HPV-16E6癌基因通过siRNA改变HPV-16E6基因在HFK和E6基因敲除阳性细胞系中的miRNA表达。在HPV-16存在的情况下差异表达的miRNAs可能为SCCHN提供生物标志物，并识别该病毒靶向的细胞通路。
Human papillomavirus (HPV) positive cases of squamous cell carcinoma of the head and neck (SCCHN) have a much better disease outcome compared to SCCHN cases lacking HPVs. Differences in microRNA (miRNA) expression may affect their clinical outcomes. miRNA expression was studied using microarrays and quantitative RT-PCR in HPV-16 positive and HPV-negative SCCHN cell lines. The role of HPV-16 E6 and E7 oncogenes in altering miRNA expression was investigated using human foreskin keratinocytes (HFKs). MiRNAs miR-363, miR-33 and miR-497 were upregulated while miR-155, miR-181a, miR-181b, miR-29a, miR-218, miR-222, miR-221 and miR-142-5p were downregulated in HPV-positive cells compared to both HPV-negative SCCHN and normal oral keratinocytes. HPV-16 E6 oncogene altered miRNA expression in HFKs and in an HPV-16 positive cell line with E6 knockdown using siRNA. MiRNAs differentially expressed in the presence of HPV-16 may provide biomarkers for SCCHN and identify cellular pathways targeted by this virus.
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影响因子：
6.4
作者：
Chang, Steven S.;Jiang, Wei Wen;Smith, Ian;Poeta, Luana M.;Begum, Shahnaz;Glazer, Chad;Shan, Shannon;Westra, William;Sidransky, David;Califano, Joseph A.
通讯作者：
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影响因子：
8.4
作者：
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通讯作者：
Khan, S
DOI：
10.1002/hed.10274
发表时间：
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影响因子：
2.9
作者：
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通讯作者：
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影响因子：
11.2
作者：
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通讯作者：
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影响因子：
64.8
作者：
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通讯作者：
Hannon, Gregory J.