Lysobacter concretionis sp. nov., isolated from anaerobic granules in an upflow anaerobic sludge blanket reactor
Lysobacter concretionis sp. nov., isolated from anaerobic granules in an upflow anaerobic sludge blanket reactor
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DOI：
10.1099/ijs.0.63399-0
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发表时间：
2005-05-01
影响因子：
2.8
通讯作者：
Lee, ST
中科院分区：
文献类型：
--
作者：
Bae, HS;Im, WT;Lee, ST
根据多相分类法的结果，对已发表的溶杆菌属（Lysobacter）和从处理啤酒废水的上流式厌氧污泥床反应器中分离到的一株新菌株Ko 07（11：0）（T）的分类地位进行了重新估计。基于16 S rRNA基因序列的系统发育推断表明，菌株Ko 07 T和所有已发表名称的溶杆菌属物种一起聚集在“γ-变形菌”纲的系统发育分支中。菌株Ko 07 T与已建立的溶杆菌属模式菌株的序列相似性在94.9- 96.7%之间。泛醌Q-8和支链脂肪酸C）（iso，C）（15：0）（iso，C）（16：0）（iso，iso C）（17：1）（w 9 c和C）（11：0）iso 3OH主要出现在菌株Ko 07 T以及公认溶杆菌属的所有模式菌株中。菌株Ko 07 T与已知溶杆菌属的DNA-DNA杂交值为2- 20%。Ko 07 T菌株在一些重要的表型特征如滑动运动、长杆状和蛋白水解活性等方面具有共同的分类学特征，但由于其较低的DNA-DNA杂交值、相对较低的DNA G + C含量（63.8mol%）、底物利用率和一些理化特性，可与已发表的溶杆菌属菌株区分开来。根据本研究的结果，建议将菌株Ko 07 T归类为溶杆菌属的一个新成员，并将其命名为Lysobacter concentionis sp. nov.。模式菌株为Ko 07 T（= KCTC 12205 T = DSM 16239 T）。
The taxonomic positions of Lysobacter species with validly published names and a novel strain Ko07(11:0)(T, which was newly isolated from an upflow anaerobic sludge blanket reactor treating wastewater from a brewery, were (re)estimated on the basis of results obtained by using a polyphasic taxonomy approach. Phylogenetic inference based on 16S rRNA gene sequences showed that strain Ko07T and all Lysobacter species with validly published names clustered together in a phylogenetic branch within the class 'Gammaproteobacteria'. The sequence similarity of strain Ko07T to the type strains of established Lysobacter species was in the range 94.9-96.7%. Ubiquinone Q-8 and branched fatty acids, C) (iso, C)(15:0) (iso, C)(16:0) (iso, iso C)(17:1) (w9c and C)(11:0) iso 3OH, predominantly appeared in strain Ko07T as well as in all type strains of the recognized Lysobacter species. The DNA-DNA hybridization values of strain Ko07T with those of recognized Lysobacter species were estimated to be 2-20 %. Despite sharing common taxonomic features in important phenotypic characteristics, such as gliding movement, long-rod shape and proteolytic activity, strain Ko07T could be distinguished from the Lysobacter species with validly published names by its low DNA-DNA hybridization value, a comparatively low DNA G + C content (63.8 mol%), substrate utilization and some physiochemical characteristics. On the basis of the results obtained in this study, it is proposed that strain Ko07T should be classified as representing a novel member of the genus Lysobacter, for which the name Lysobacter concretionis sp. nov. is proposed. The type strain is Ko07T (= KCTC 12205T = DSM 16239T).