Dendritic diversification through transcription factor-mediated suppression of alternative morphologies
Dendritic diversification through transcription factor-mediated suppression of alternative morphologies
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DOI：
10.1242/dev.130906
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发表时间：
2016-04-15
期刊：
影响因子：
4.6
通讯作者：
Grueber, Wesley B.
中科院分区：
文献类型：
--
作者：
Corty, Megan M.;Tam, Justina;Grueber, Wesley B.
神经元表现出惊人的功能和形态多样性，而多样化背后的发育机制对于理解神经回路组装和功能具有重要意义。我们发现果蝇感觉神经元的形态通过一系列抑制性转录相互作用而多样化，这些抑制性转录相互作用涉及POU域转录因子Pdm1（Nubbin）和Pdm2、同源域转录因子Cut以及转录调节因子Scalloped和Vestigial。 Pdm1 和 Pdm2 在本体感觉神经元的子集中表达，并具有抑制树突生长和分支的功能。触摸感受器的一个子集显示出表达 Pdm1/2 的能力，但 Cut 会抑制这种表达并促进更复杂的树突乔木。通过选择性表达 Scalloped 和 Vestigial，Cut 表达水平在不同的感觉神经元中多样化。不同水平的 Cut 会影响树突的复杂性，与此一致，我们表明 Scalloped 和 Vestigial 会抑制末端树突分支。因此，这种转录层次结构可以抑制替代形态，从而使三种不同类型的体感神经元多样化。
Neurons display a striking degree of functional and morphological diversity, and the developmental mechanisms that underlie diversification are of significant interest for understanding neural circuit assembly and function. We find that the morphology of Drosophila sensory neurons is diversified through a series of suppressive transcriptional interactions involving the POU domain transcription factors Pdm1 ( Nubbin) and Pdm2, the homeodomain transcription factor Cut, and the transcriptional regulators Scalloped and Vestigial. Pdm1 and Pdm2 are expressed in a subset of proprioceptive sensory neurons and function to inhibit dendrite growth and branching. A subset of touch receptors show a capacity to express Pdm1/2, but Cut represses this expression and promotes more complex dendritic arbors. Levels of Cut expression are diversified in distinct sensory neurons by selective expression of Scalloped and Vestigial. Different levels of Cut impact dendritic complexity and, consistent with this, we show that Scalloped and Vestigial suppress terminal dendritic branching. This transcriptional hierarchy therefore acts to suppress alternative morphologies to diversify three distinct types of somatosensory neurons.