In vivo administration of monoclonal antibody to the NK 1.1 antigen of natural killer cells: effect on acute murine cytomegalovirus infection.
In vivo administration of monoclonal antibody to the NK 1.1 antigen of natural killer cells: effect on acute murine cytomegalovirus infection.
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体内施用针对自然杀伤细胞 NK 1.1 抗原的单克隆抗体：对急性鼠巨细胞病毒感染的影响。
DOI：
10.1002/jmv.1890300113
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发表时间：
1990
影响因子：
12.7
通讯作者：
Shanley,JD
中科院分区：
文献类型：
--
作者：
Shanley,JD
在许多品系小鼠的自然杀伤细胞上发现了针对 NK 1.1 抗原的单克隆抗体，在体内给药时会特异性抑制 NK 细胞功能。使用这种单克隆抗体，我们研究了体内自然杀伤 (NK) 细胞的特异性抑制对 C57BL/10ScN 小鼠急性鼠巨细胞病毒 (MCMV) 感染的影响。给予 NK 1.1 抗体显着降低了 C57BL/10ScN 小鼠对致命病毒攻击的抵抗力。此外，腹膜内感染前施用抗体显着增加唾液腺、肺和脾中的 MCMV 复制。在C3H/HeN小鼠中，缺乏NK 1.1抗原的品系、NK 1.1抗体对病毒复制或致死感染没有影响。因此，体内施用针对 NK 1.1 的单克隆抗体可改变急性 MCMV 感染的进程。这些发现进一步证实了 NK 细胞在防御急性 MCMV 感染中的作用。
Monoclonal antibody to the NK 1.1 antigen, found on the natural killer cells of a number of strains of mice, specifically suppresses NK cell function when given in vivo. Using this monoclonal antibody, we have examined the effects of specific suppression of natural killer (NK) cells in vivo on acute murine cytomegalovirus (MCMV) infection in C57BL/10ScN mice. Administration of antibody to NK 1.1 substantially lowered the resistance of C57BL/10ScN mice to lethal virus challenge. In addition, antibody administration prior to intraperitoneal infection significantly increased MCMV replication in salivary glands, lungs, and spleens. In C3H/HeN mice, a strain that lacks the NK 1.1 antigen, antibody to NK 1.1 had no effect on virus replication or lethal infection. Thus, in vivo administration of monoclonal antibody to NK 1.1 alters the course of acute MCMV infection. These findings further substantiate the role of NK cells in defense against acute MCMV infection.