Permeation of small molecules into the cavity of ferritin as revealed by proton nuclear magnetic resonance relaxation.
Permeation of small molecules into the cavity of ferritin as revealed by proton nuclear magnetic resonance relaxation.
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DOI：
10.1042/bj3070253
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发表时间：
1995-04
期刊：
影响因子：
--
通讯作者：
D. Yang;K. Nagayama
中科院分区：
文献类型：
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作者：
D. Yang;K. Nagayama
利用核磁共振弛豫技术研究了分子在铁蛋白腔内的渗透。测定了不含铁的重组马l -铁铁蛋白溶液和含极少量铁离子的马脾铁铁蛋白溶液在不同探针分子旋转框架内的自旋晶格弛豫时间。结果表明，大于铁蛋白通道大小的分子可以通过通道进入铁蛋白内部，而分子的最大渗透尺寸小于麦芽糖。
The NMR relaxation technique was used to investigate the permeation of molecules into the cavity of ferritin. Spin-lattice relaxation times in the rotating frame of various probe molecules were measured for solutions of recombinant horse L-apoferritin without iron and horse spleen apoferritin with very small amounts of ferric ions. The results show that molecules larger than the size of the ferritin channels can pass through the channels into the ferritin interior, and that the maximum size of molecules for the permeation is smaller than maltotriose.