Chlorpromazine and skeletal muscle: A study of skinned single fibers of the guinea pig
Chlorpromazine and skeletal muscle: A study of skinned single fibers of the guinea pig
复制标题
氯丙嗪和骨骼肌：豚鼠去皮单纤维的研究
DOI：
10.1016/0014-4886(81)90281-8
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发表时间：
1981
影响因子：
5.3
通讯作者：
A. Takagi
中科院分区：
文献类型：
--
作者：
A. Takagi
本文用带皮单纤维标本观察了氯丙嗪（CPZ）对豚鼠快收缩肌的作用。药物影响收缩系统和肌浆网（SR）的功能。CPZ使收缩系统对钙离子更敏感，使张力-pCa曲线向左移动，但它不改变在饱和Ca浓度下产生的最大张力。CPZ引起收缩的纤维释放钙离子的SR。钙释放由CPZ抑制普鲁卡因或镁离子，并促进当介质中含有更多的游离钙离子。这些观察结果表明，钙释放CPZ是由于激活钙诱导的钙释放机制。CPZ对SR的Ca转运的影响及其通过Mg离子的修饰在三磷酸腺苷的存在下以及在其存在下是相当相等的。这表明CPZ的主要作用部位是在Ca释放机制中。CPZ浓度大于0.3mM时，SR功能受到不可逆的损害。
The effect of chlorpromazine (CPZ) on fast twitch muscle of the guinea pig was examined by using the skinned single-fiber preparation. The drug influenced the function of both the contractile system and the sarcoplasmic reticulum (SR). CPZ made the contractile system more sensitive to calcium ion, shifting the tension-pCa curve to the left, but it did not alter the maximal tension developed at a saturating concentration of Ca. CPZ elicited contracture of the fiber by releasing Ca ion from the SR. Calcium release by CPZ was inhibited either by procaine or magnesium ion and was facilitated when the medium contained more free Ca ion. These observations suggested that Ca release by CPZ was due to the activation of a Ca-induced Ca release mechanism. The effect of CPZ on Ca transport of the SR and its modification by Mg ion was quite equal in the absence of adenosine triphosphate as well as its presence. This indicates that the main site of action of CPZ is in the Ca release mechanism. A CPZ concentration greater than 0.3 mmdamaged the function of the SR irreversibly.