METABOLIC AND SCINTIGRAPHIC STUDIES OF RADIOIODINATED HUMAN C-REACTIVE PROTEIN IN HEALTH AND DISEASE
METABOLIC AND SCINTIGRAPHIC STUDIES OF RADIOIODINATED HUMAN C-REACTIVE PROTEIN IN HEALTH AND DISEASE
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DOI：
10.1172/jci116336
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发表时间：
1993-04-01
影响因子：
15.9
通讯作者：
HAWKINS, PN
中科院分区：
文献类型：
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作者：
VIGUSHIN, DM;PEPYS, MB;HAWKINS, PN
对8例健康对照者和35例住院患者（包括类风湿性关节炎、系统性红斑狼疮、感染和肿瘤）进行了血浆和全身C-反应蛋白（CRP）的转换研究。I-125-CRP的血浆清除率接近单指数函数，并且在对照组和所有患者组中相似。没有证据表明在任何研究的疾病中CRP的加速清除或催化。19小时的半衰期比以前研究的大多数人血浆蛋白更快，并且分解率与血浆CRP浓度无关。因此，CRP的合成速率是其血浆水平的唯一重要决定因素，证实了血清CRP测量作为急性期反应相关疾病中疾病活动性的客观指标的有效性。约90%的注射放射性7天后在尿液中回收，和与I-123标记的CRP在10例不同的局灶性病理学的放射性成像研究显示没有显着的定位示踪剂。因此，CRP的功能可能主要在流体相中而不是通过在组织损伤或炎症部位的主要沉积来实现。
Plasma and whole-body turnover studies of human C-reactive protein (CRP), isolated from a single normal healthy donor and labeled with I-125, Were undertaken in 8 healthy control subjects and 35 hospitalized patients including cases of rheumatoid arthritis, systemic lupus erythematosus, infections, and neoplasia. Plasma clearance of I-125-CRP closely approximated to a monoexponential function and was similar in the control and all patient groups. There was no evidence for accelerated clearance or catabolism of CRP in any of the diseases studied. The 19-h half-life was more rapid than that of most human plasma proteins studied previously, and the fractional catabolic rate was independent of the plasma CRP concentration. The synthesis rate of CRP is thus the only significant determinant of its plasma level, confirming the validity of serum CRP measurement as an objective index of disease activity in disorders associated with an acute-phase response. Approximately 90% of injected radioactivity was recovered in the urine after 7 d, and scintigraphic imaging studies with I-123-labeled CRP in 10 patients with different focal pathology showed no significant localization of tracer. The functions of CRP are thus likely to be effected predominantly in the fluid phase rather than by major deposition at sites of tissue damage or inflammation.