Diversity of MICA (PERB11.1) and HLA haplotypes in Northeastern Thais
Diversity of MICA (PERB11.1) and HLA haplotypes in Northeastern Thais
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DOI：
10.1034/j.1399-0039.2001.580203.x
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发表时间：
2001-08-01
期刊：
影响因子：
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通讯作者：
Inoko, H
中科院分区：
文献类型：
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作者：
Romphruk, AV;Naruse, TK;Inoko, H
MICA 或 PERB11.1 是一种多态性主要组织相容性复合体 (MHC) I 类相关基因，位于 HLA I 类区域中 HLA-B 基因的 46 kb 着丝粒处。它主要在肠道上皮细胞、角质形成细胞、内皮细胞、成纤维细胞和单核细胞中表达，并因热应激而上调。研究发现 NUCA 可以与 γδT 细胞、CD8+ 细胞以及携带 NKG2D/DAP10 受体的自然杀伤 (NK) 细胞相互作用。 MICA 基因表现出高度多态性，至少有 54 个等位基因。在本研究中，使用基于测序的分型 (SBT) 对 255 名无关的健康泰国东北部人的 MICA 基因的多态性外显子 2、3 和 4 进行了分析。检测到 13 个先前报道的 MICA 等位基因。 MICA*008、*010、*002 和*019 具有高度优势，等位基因频率分别为 21.4%、18.2%、17.6% 和 15.3%。这 13 个 MICA 等位基因中的 5 个显示出与日本人和白种人人群显着不同的频率。有趣的是，MICA052是其他人群中非常罕见的等位基因，其等位基因频率为8.2%，主要出现在该人群中携带HLA-B*13的BLA单倍型上。强连锁不平衡。在 MCA 和 HLA-B 之间观察到，与在其他人群中观察到的情况类似，即 MICA*010-B*4601、MICA052-B*13、MICA*002-B*5801 和 MICA*019-B*15 (1502、1508、1511、1515、1528、1530)。在泰国东北部人群中发现了多种三基因座（MICA - HLA-B - HLA-Cw）和六基因座（HLA-DQB1 - HLA-DRB1 - MICA - HLA-B - HLA-Cw - HLA-A）单倍型。这是第一份关于东南亚人群中 MICA 等位基因分布的报告。这些数据将为未来分析MICA基因在东南亚人群疾病易感性和移植匹配中的潜在作用提供重要依据。
MICA or PERB11.1 is a polymorphic major histocompatibility complex (MHC) class I-related gene located 46 kb centromeric of the HLA-B gene in the HLA class I region. It is expressed mainly in gut epithelial cells, keratinocytes, endothelial cells, fibroblasts and monocytes, and is upregulated by heat stress. NUCA has been found to interact with gamma delta T cells, up CD8(+) and natural killer (NK) cells bearing the NKG2D/DAP10 receptor. The MICA gene displays a high degree of polymorphism with at least 54 alleles. In the present study, polymorphic exons 2, 3 and 4 of the MICA gene were analyzed using sequencing based typing (SBT) in 255 unrelated healthy northeastern Thais. Thirteen previously reported MICA alleles were detected. MICA*008, *010, *002 and *019 Were highly predominant with the allele frequencies of 21.4%, 18.2%, 17.6% and 15.3%, respectively. Five of these 13 MICA alleles show significantly different frequencies from those of the Japanese and Caucasian populations. Interestingly, MICA052, which is a very rare allele in other populations, was prevalent with the allele frequency of 8.2%, mainly on the BLA haplotype carrying HLA-B*13 in this population. Strong linkage disequilibria. were observed between MCA and HLA-B, as similarly observed in other populations, namely MICA*010-B*4601, MICA052-B*13, MICA*002-B*5801, and MICA*019-B*15 (1502, 1508, 1511, 1515, 1528, 1530). A large variety of three-locus (MICA - HLA-B - HLA-Cw) and six-locus (HLA-DQB1 - HLA-DRB1 - MICA - HLA-B - HLA-Cw - HLA-A) haplotypes were recognized in the northeastern Thai population. This is the first report on MICA allelic distribution in Southeast Asian populations. These data will provide the important basis for future analyses on the potential role of the MICA gene in disease susceptibility and transplantation matching in Southeast Asian populations.