Structure of the Core oligosaccharide from the lipopolysaccharide of Pseudomonas aeruginosa PAC1R and its defective mutants.
Structure of the Core oligosaccharide from the lipopolysaccharide of Pseudomonas aeruginosa PAC1R and its defective mutants.
复制标题
铜绿假单胞菌 PAC1R 及其缺陷突变体脂多糖的核心寡糖的结构。
DOI：
10.1111/j.1432-1033.1983.tb07366.x
复制
发表时间：
1983
期刊：
影响因子：
--
通讯作者：
P. Meadow
中科院分区：
文献类型：
--
作者：
P. Rowe;P. Meadow
从铜绿假单胞菌 PAC1R 及其脂多糖缺陷突变体中分离出脂多糖。通过使用 Bio-Gel P6 的凝胶色谱法，在弱酸水解后从中获得核心级分。来自亲本菌株的核心部分含有葡萄糖、鼠李糖、半乳糖胺、庚糖和丙氨酸，摩尔比大约为3:1:1:1:1，而来自缺陷突变体的核心部分缺乏鼠李糖和/或一个、两个或三个葡萄糖单位。核心组分的甲基化分析，包括通过气液色谱和质谱相结合鉴定部分甲基化的糖醇乙酸酯，可以制定可能的结构。这些均通过 13C 核磁共振波谱证实。从亲本菌株和突变体之一中分离出由附着有未聚合的O-抗原的核心组成的级分，并以相同的方式进行分析以鉴定O-抗原的附着点。
综合数据得出 PAC1R 核心寡糖及其突变体的配方如下：
Lipopolysaccharides were isolated from Pseudomonas aeruginosa PAC1R and its lipopolysaccharide-defective mutants. Core fractions were obtained from them following mild acid hydrolysis, by gel chromatography using Bio-Gel P6. The core fraction from the parent strain contained glucose, rhamnose, galactosamine, heptose and alanine in the approximate molar ratios 3:1:1:1:1 while core fractions from the defective mutants lacked rhamnose and/or one, two, or three glucose units. Methylation analysis of the core fractions including identification of the partially methylated alditol acetates by combined gas-liquid chromatography and mass spectroscopy allowed the formulation of possible structures. These were confirmed by 13C nuclear magnetic resonance spectroscopy. Fractions consisting of core to which was attached unpolymerised O-antigen were isolated from the parent strain and one of the mutants and analysed in the same way to identify the attachment point of the O-antigen.
The combined data leads to the formulation of the core oligosaccharide of PAC1R and its mutants as follows: