Comparison of the cytotoxic effects of merocyanine-540 on leukemic cells and normal human bone marrow.
Comparison of the cytotoxic effects of merocyanine-540 on leukemic cells and normal human bone marrow.
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DOI：
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发表时间：
1986-10
期刊：
影响因子：
11.2
通讯作者：
J. Atzpodien;S. Gulati;B. Clarkson
中科院分区：
文献类型：
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作者：
J. Atzpodien;S. Gulati;B. Clarkson
已经描述了各种化学化合物诱导肿瘤细胞的光敏化，导致细胞死亡。我们研究了Mercury-540（MC-540）对白血病和正常骨髓（BM）细胞的作用。将急性早幼粒细胞白血病（HL-60）和普通急性淋巴细胞白血病抗原阳性的非T、非B急性淋巴细胞白血病（Reh）细胞系与MC-540一起孵育，同时暴露于白色光。在类似条件下处理正常人BM和白血病细胞与BM细胞的混合物。在50，000 lx的恒定照明率下，对于HL-60，MC-540浓度为20微克/ml或更高，对于Reh细胞，MC-540浓度为10微克/ml或更高，获得显著的（至少4至5 log）肿瘤细胞破坏。在同等条件下孵育BM保留了18.0%的粒细胞-巨噬细胞集落形成单位和14.2%的红细胞爆发形成单位。当肿瘤细胞与照射的BM混合，然后用MC-540处理时，获得了类似的结果。总之，MC-540的细胞光敏化对白血病细胞具有选择性细胞毒性作用，因此可用于从自体BM中清除肿瘤细胞。
Various chemical compounds have been described to induce photosensitization of tumor cells resulting in cell death. We studied the effect of merocyanine-540 (MC-540) on both leukemic and normal bone marrow (BM) cells. Acute promyelocytic leukemia (HL-60) and common acute lymphoblastic leukemia antigen-positive non-T, non-B acute lymphoblastic leukemia (Reh) cell lines were incubated with MC-540 and simultaneously exposed to white light. Normal human BM and mixtures of leukemic cells with BM cells were treated under similar conditions. At constant illumination rates of 50,000 lx, significant (at least 4 to 5 logs) tumor cell destruction was obtained with MC-540 concentrations of 20 micrograms/ml or more for HL-60, and 10 micrograms/ml or more for Reh cells. Incubation of BM under equivalent conditions preserved 18.0% of granulocyte-macrophage colony-forming units and 14.2% of erythroid burst-forming units. Similar results were obtained when tumor cells were mixed with irradiated BM and then treated with MC-540. In summary, cell photosensitization with MC-540 has a selective cytotoxic effect towards leukemic cells and therefore may be useful for purging tumor cells from autologous BM.