Elevated Serum Interleukin 33 Is Associated with Autoantibody Production in Patients with Rheumatoid Arthritis.
Elevated Serum Interleukin 33 Is Associated with Autoantibody Production in Patients with Rheumatoid Arthritis.
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DOI：
10.3899/jrheum.100184
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发表时间：
2010-10-01
影响因子：
3.9
通讯作者：
Li, Zhan-Guo
中科院分区：
文献类型：
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作者：
Mu, Rong;Huang, He-Qing;Li, Zhan-Guo
目标。白细胞介素33 （IL-33）是一种在动物模型中参与关节炎症的新型细胞因子。分析IL-33在类风湿关节炎（RA）患者血清和滑液中的表达，探讨其可能的病理生理意义。采用ELISA法检测223例RA患者和159例对照组血清中IL-33的浓度。ELISA法检测抗环瓜氨酸肽、类风湿因子(RF)-IgA、RF- igg。间接免疫荧光法检测抗角蛋白抗体和抗核周因子。红细胞沉降率、c反应蛋白和免疫球蛋白用标准实验室技术测定。分析IL-33水平与RA临床及血清学特征的关系。我们检测了40例ra患者肿瘤坏死因子阻断治疗后IL-33水平的变化。与骨关节炎和健康血清中几乎无法检测到IL-33相比，223例RA患者中有94例（42.2%）可检测到IL-33。RA患者血清IL-33浓度明显高于对照组。抗tnf治疗后血清IL-33水平降低。血清IL-33水平与IgM和ra相关自身抗体的产生相关，包括RF和抗纤氨酸化蛋白抗体。然而，IL-33浓度与急性期炎症反应物及疾病活动指数评分之间没有相关性，提示IL-33与ra炎症之间的联系具有复杂性或间接性。RA血清中IL-33水平异常升高。血清IL-33的升高至少部分归因于RA中过量的tnf - α。IL-33可能参与RA自身抗体产生的调控。（2010年8月1日首次发布；journal Rheumatol 2010; 37:06 -13; doi:10.3899/ jrheumat. 100184）
Objective. Interleukin 33 (IL-33) is a novel cytokine involved in joint inflammation in animal models. We analyzed the expression of IL-33 in the serum and synovial fluid of patients with rheumatoid arthritis (RA) and investigated its possible pathophysiological importance.Methods. The concentration of IL-33 was measured by ELISA in the serum of 223 patients with RA and 159 controls. Anticyclic citrullinated peptide, rheumatoid factor (RF)-IgA, and RF-IgG were tested by ELISA. Antikeratin antibody and antiperinuclear factor were tested by indirect immunofluorescence assay. Erythrocyte sedimentation rate, C-reactive protein, and immunoglobulins were measured by standard laboratory techniques. The association of IL-33 level with clinical and serologic features of RA was analyzed. We tested the change of IL-33 level following tumor necrosis factor (TNF-alpha) blockade therapy in 40 patients with RA.Results. In contrast to almost no detectable IL-33 in osteoarthritis and healthy serum, IL-33 could be detected in 94 out of the 223 RA cases (42.2%). Serum IL-33 concentration was significantly higher in patients with RA than in control groups. The level of serum IL-33 decreased after anti-TNF treatment. The level of serum IL-33 was correlated with the production of IgM and RA-related autoantibodies including RF and anticitrullinated protein antibodies. However, no correlation was found between IL-33 concentration and acute-phase inflammation reactant or the score of the Disease Activity Index, suggesting a complex or indirect character of the link between IL-33 and the inflammation in RA.Conclusion. The level of IL-33 is abnormally elevated in RA serum. The elevation of serum IL-33 was at least partly attributed to excessive TNF-alpha in RA. IL-33 might be involved in the regulation of autoantibody production in RA. (First Release August I 2010; J Rheumatol 2010;37:2006-13; doi:10.3899/jrheum.100184)