Fluorescence correlation spectroscopy at high concentrations using gold bowtie nanoantennas
Fluorescence correlation spectroscopy at high concentrations using gold bowtie nanoantennas
复制标题
DOI：
10.1016/j.chemphys.2012.04.011
复制
发表时间：
2012-10-08
期刊：
影响因子：
2.3
通讯作者：
Moerner, W. E.
中科院分区：
文献类型：
--
作者：
Kinkhabwala, Anika A.;Yu, Zongfu;Moerner, W. E.
荧光相关光谱(FCS)测量溶液中荧光团的荧光波动，但仅限于极低浓度。与未增强分子的大背景相比，等离子体金蝴蝶结纳米天线增强了单个分子的荧光，这里我们展示了蝴蝶结可以将FCS测量扩展到更高的浓度。在这个演示中，蝴蝶结-FCS信号主要是由在蝴蝶结间隙附近瞬间附着在衬底上的分子主导的，并且在10-100ms的时间尺度上测量了两个荧光团的光漂白/光闪烁动力学。(C)2012爱思唯尔B.V.保留所有权利。
Fluorescence correlation spectroscopy (FCS) measures the fluorescence fluctuations of fluorophores in solution, but is restricted to extremely low concentrations. Plasmonic gold bowtie nanoantennas enhance a single molecule's fluorescence relative to a large background of unenhanced molecules, and here we show that bowties can extend FCS measurements to much higher concentrations. In this demonstration, the bowtie-FCS signal is dominated by molecules that transiently stick to the substrate near the bowtie gap, and photobleaching/photoblinking dynamics for two fluorophores are measured on the 10-100 ms time scale. (C) 2012 Elsevier B. V. All rights reserved.