A Quantitative Method for Determining Uptake of Silica Nanoparticles in Macrophages by Single Particle Inductively Coupled Plasma-Mass Spectrometry.
A Quantitative Method for Determining Uptake of Silica Nanoparticles in Macrophages by Single Particle Inductively Coupled Plasma-Mass Spectrometry.
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DOI：
10.1002/cpz1.396
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发表时间：
2022-03
期刊：
影响因子：
--
通讯作者：
Brown JM
中科院分区：
文献类型：
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作者：
Rogers KL;Cruz-Hernandez A;Brown JM
工程纳米材料在我们的社会中正变得越来越普遍，在医学、消费品、生物修复和先进材料方面有着广泛的应用。随着这些纳米材料种类的增多，分析它们的特性具有重要意义。单粒子电感耦合等离子体质谱(SP-ICP-MS)是一种高通量、灵敏、可靠的仪器分析方法，用于同时表征和定量各种基质中的纳米颗粒。其中一种感兴趣的纳米颗粒是无定形二氧化硅纳米颗粒(SiNPs)。SiNPs在食品和化妆品等消费品中有着广泛的应用，是新型医疗应用和环境生物修复的主要候选者。尽管它们的使用越来越多，但SiNPs在体外和体内都被证明具有毒理学特性，特别是在免疫系统方面。由于在普通公众和职业环境中可能增加SiNP的暴露，研究SiNP与巨噬细胞等免疫细胞的关系对于阐明毒性机制至关重要。为了有效地确定纳米颗粒的毒性，关键是要检查剂量学和目标细胞所摄取的纳米颗粒的量。不同的细胞类型有不同的摄取曲线，不同的物理化学性质支配着纳米粒子的剂量测定和细胞摄取。在这里，我们描述了一种使用SP-ICPMS来量化和表征细胞和介质样本中存在的SiNPs的大小、大小分布和数量的方法。我们使用一步消化法，这允许消化生物基质，同时保持SiNPs的完整性，用于SP-ICPMS分析。临床上，这种方法有可能被用作分析其他生物基质中的SiNP的方法，潜在地作为一种将SiNP摄取定义为免疫介导性疾病的生物标记物的方法。
Engineered nanomaterials are becoming increasingly ubiquitous in our society, with numerous applications in medicine, consumer products, bioremediation, and advanced materials. As these nanomaterials increase in variety, analyzing their characteristics is of great importance. Single particle inductively coupled plasma- mass spectrometry (SP-ICP-MS) is a high throughput, sensitive, and robust instrumental analysis method used to simultaneously characterize and quantify nanoparticles in a variety of matrices. One such type of nanoparticle of interest are amorphous silica nanoparticles (SiNPs). SiNPs have widespread use in consumer products such as food and cosmetics, and are prime candidates for novel medical applications and uses in environmental bioremediation. Despite their increased use, SiNPs have been shown to have toxicological properties in vitro and in vivo, particularly with regard to the immune system. Because of the potential for increased SiNP exposure to the general public and in occupational settings, examining the relationship SiNPs have with immune cells such as macrophages, is vital to elucidating mechanisms of toxicity. To effectively determine the toxicity of nanoparticles, it is critical to examine dosimetry and the amount of nanoparticle taken up by the cell of interest. Different cell types have different uptake profiles, and varying physicochemical properties govern nanoparticle dosimetry and uptake in cells. Here, we describe a protocol using SP-ICP-MS to quantify and characterize the size, size distribution, and amount of SiNPs present in a cell and media sample. We use a single-step digestion, which allows for the digestion of biological matrices while simultaneously keeping the SiNPs intact for SP-ICP-MS analysis. Clinically, this approach has potential to be used as a method for analyzing SiNPs in other biological matrices, potentially as a way of defining SiNP uptake as a biomarker in immune-mediated diseases.