A protocol for conducting 7-day daily renewal tests with Lemna gibba
A protocol for conducting 7-day daily renewal tests with Lemna gibba
复制标题
DOI：
10.1038/nprot.2007.146
复制
发表时间：
2007-01-01
期刊：
影响因子：
14.8
通讯作者：
Solomon, Keith R.
中科院分区：
文献类型：
--
作者：
Brain, Richard A.;Solomon, Keith R.
浮萍（Lemna gibba）是一种常用于毒性测试的淡水水生植物，并且浮萍属（Lemna spp）是目前唯一需要根据EPA农药登记指南进行农药评估的水生高等植物。包括美国材料试验学会、经合组织、环境保护局和加拿大环境部在内的各组织和机构目前可用于毒性测试的方法主要是静态或半静态测试，更新间隔未作规定（经合组织），由于有毒物质在水生介质中的半衰期短、植物吸收化学品以及营养介质蒸发，可能无法提供一致的接触方法。本文所述的程序详细描述了一种简单有效的7天每日静态更新程序，用于进行L. gibba，评估的适当终点，分析毒性数据所需的统计标准，以及培养和维持L. gibba该协议基于广泛接受的静态方法的修改版本。
Lemna gibba (a duckweed) is a freshwater macrophyte commonly used in toxicity testing, and Lemna spp are currently the only aquatic higher plants required for evaluation of pesticides under the pesticide registration guidelines of the EPA. The methods currently available for toxicity testing by various organizations and agencies, including ASTM, OECD, EPA and Environment Canada, are largely static or semistatic tests with unspecified renewal intervals (OECD) and may not provide a consistent means of exposure owing to short toxicant half-life in aquatic media, uptake of chemical by plants and evaporation of nutrient media. The procedure outlined here details a simple and efficient 7-day daily static renewal procedure for conducting toxicity tests with L. gibba, the appropriate end points to assess, the statistical criteria necessary for analyzing the toxicity data, as well as the steps required to culture and maintain L. gibba. This protocol is based on a modified version of a widely accepted static method.