Maternal diabetes in vivo and high glucose concentration in vitro increases apoptosis in rat embryos
Maternal diabetes in vivo and high glucose concentration in vitro increases apoptosis in rat embryos
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DOI：
10.1016/j.reprotox.2006.08.009
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发表时间：
2007-01-01
影响因子：
3.3
通讯作者：
Wentzel, Patti
中科院分区：
文献类型：
--
作者：
Gaereskog, Mattias;Cederberg, Jonas;Wentzel, Patti
细胞凋亡可能与糖尿病引起的胚胎畸形发生有关。我们评估了糖尿病妊娠大鼠模型胚胎中细胞凋亡的发生。我们发现 Bcl-2 减少。糖尿病大鼠胚胎中 Bax 和裂解 Caspase 3 蛋白的增加。此外，我们发现，与对照胚胎的细胞相比，先前暴露于糖尿病样环境（体内、体外）的胚胎细胞中 Caspase 3 的激活增加，通过补充 N-乙酰半胱氨酸或凋亡抑制剂使其正常化。我们检测到暴露于母体糖尿病的胚胎细胞中碘化丙啶的摄取增加，这一发现经活体染色证实。此外，我们发现体内和体外暴露于糖尿病环境的胚胎的畸形发生增加。器官形成期间暴露于糖尿病环境会增加胚胎细胞的凋亡和胚胎的畸形发生。细胞凋亡率增加可能通过诱导胚胎成熟紊乱、吸收率增加和先天畸形而在糖尿病胚胎病中发挥作用。 (c) 2006 Elsevier Inc. 保留所有权利。
Apoptosis may be involved in diabetes-induced embryonic dysmorphogenesis. We estimated the occurrence of apoptosis in embryos of a rat model for diabetic pregnancy.We found decreased Bcl-2. increased Bax and cleaved Caspase 3 proteins in embryos from diabetic rats. Moreover, we found increased activation of Caspase 3 in cells from embryos previously exposed to a diabetes-like environment (in vivo, in vitro) compared to cells from control embryos, which was normalized by supplementation of N-acetylcysteine or apoptosis inhibitor. We detected increased propidium iodide uptake in embryonic cells exposed to maternal diabetes, a finding confirmed by vital staining. Additionally, we found increased dysmorphogenesis in embryos exposed to a diabetic environment in vivo and in vitro.Exposure to a diabetic milieu during organogenesis increases apoptosis in embryonic cells and dysmorphogenesis in embryos. Enhanced apoptotic rate may have a role in diabetic embryopathy by inducing disturbed embryonic maturation, increased rates of resorptions and congenital malformations. (c) 2006 Elsevier Inc. All rights reserved.