Up-regulation of genes related to the ubiquitin-proteasome system in the brown adipose tissue of 24-h-fasted rats
Up-regulation of genes related to the ubiquitin-proteasome system in the brown adipose tissue of 24-h-fasted rats
复制标题
DOI：
10.1271/bbb.70508
复制
发表时间：
2008-01-01
影响因子：
1.6
通讯作者：
Abe, Keiko
中科院分区：
文献类型：
--
作者：
Nakai, Yuji;Hashida, Hiroko;Abe, Keiko
棕色脂肪组织（BAT）和白色脂肪组织（WAT）之间的功能平衡对于代谢稳态是重要的。我们通过DNA微阵列分析比较了禁食对BAT、WAT和肝脏基因表达谱的影响。从喂食或禁食24 h的大鼠中获得组织。考虑到错误发现率，我们提取了在进食和禁食大鼠之间差异表达的前1,000个基因。在所有三种组织中，基因本体分析显示，脂质和蛋白质生物合成相关基因已显着下调。还观察到全身燃料从葡萄糖转移到三酰甘油和自噬的诱导。有显着上调的基因在'蛋白质泛素化'类别，特别是在BAT的禁食大鼠，这表明泛素-蛋白酶体系统参与节省能源作为一种适应食物短缺。
The functional balance between brown adipose tissue (BAT) and white adipose tissue (WAT) is important for metabolic homeostasis. We compared the effects of fasting on the gene expression profiles in BAT, WAT and liver by using a DNA microarray analysis. Tissues were obtained from rats that had been fed or fasted for 24 h. Taking the false discovery rate into account, we extracted the top 1,000 genes that had been differentially expressed between the fed and fasted rats. In all three tissues, a Gene Ontology analysis revealed that the lipid and protein biosynthesis-related genes had been markedly down-regulated. The whole-body fuel shift from glucose to triacylglycerol and the induction of autophagy were also observed. There was marked up-regulation of genes in the 'protein ubiquitination' category particularly in BAT of the fasted rats, suggesting that the ubiquitin-proteasome system was involved in saving energy as an adaptation to food shortage.