Role of the renin-angiotensin-aldosterone system in the enhancement of salt sensitivity caused by prenatal protein restriction in stroke-prone spontaneously hypertensive rats
Role of the renin-angiotensin-aldosterone system in the enhancement of salt sensitivity caused by prenatal protein restriction in stroke-prone spontaneously hypertensive rats
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DOI：
10.1016/j.jnutbio.2011.04.014
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发表时间：
2012-08-01
影响因子：
5.6
通讯作者：
Murakami, Tetsuo
中科院分区：
文献类型：
--
作者：
Otani, Lila;Sugimoto, Naoya;Murakami, Tetsuo
我们之前证明，怀孕期间母体蛋白质限制会增强易发生中风的自发性高血压大鼠（SHRSP）的盐敏感性并缩短寿命。本研究的目的是调查肾素-血管紧张素-醛固酮系统在怀孕期间饲喂低蛋白饮食的母鼠后代盐敏感性发展中的参与。我们使用了在怀孕期间饲喂 20% 酪蛋白饮食 (CN) 或 9% 酪蛋白饮食 (LP) 的母鼠的 SHRSP 后代。 CN和LP SHRSP后代从出生后10周起进一步细分为饮用自来水组和饮用1%盐水组。在 LP SHRSP 后代中观察到盐负荷导致血压显着升高。 LP 后代肾脏中的盐负荷导致 CYP1182（一种醛固酮合成酶）的蛋白质水平显着升高。用醛固酮受体拮抗剂治疗 LP 后代可防止血压升高，并延长 LP 后代对饮用 1% 盐水的平均寿命。无论是饮用自来水还是饮用盐水，CN 和 LP 后代的血管紧张素转换酶活性或血管紧张素 1 型受体的蛋白质水平均未发现差异。总之，高盐负荷导致醛固酮产量的增加可能导致蛋白质限制母鼠后代对盐的敏感性升高。 (C) 2012 Elsevier Inc. 保留所有权利。
We previously demonstrated that maternal protein restriction during pregnancy enhanced salt sensitivity and shortened life span in stroke-prone spontaneously hypertensive rats (SHRSP). The present study was conducted to investigate the participation of the renin-angiotensin-aldosterone system in the development of salt sensitivity in the offspring of dams fed a low-protein diet during pregnancy. We used SHRSP offspring from dams fed a 20% casein diet (CN) or a 9% casein diet (LP) during pregnancy. The CN and LP SHRSP offspring were further subdivided into tap-water-drinking and 1%-saline-drinking groups from the postnatal 10th week. A remarkable elevation in blood pressure in response to salt loading was observed in the LP SHRSP offspring. The protein levels of CYP1182, an enzyme for aldosterone synthesis, were markedly elevated in response to salt loading in the kidneys of LP offspring. Treatment of the LP offspring with an aldosterone receptor antagonist prevented the blood pressure from elevating and lengthened the average life span in LP offspring in response to the drinking of 1% saline. No difference in the activity of angiotensin-converting enzyme or in the protein level of the angiotensin type 1 receptor was found between the CN and LP offspring in either the tap-water-drinking or saline-drinking conditions. In conclusion, the increment of aldosterone production in response to high-salt loading may contribute to the elevated salt sensitivity of the offspring of protein-restricted dams. (C) 2012 Elsevier Inc. All rights reserved.