Quantitative comparison of free fatty acids in camel, goat and cow milk

Quantitative comparison of free fatty acids in camel, goat and cow milk
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骆驼奶、山羊奶和牛奶中游离脂肪酸的定量比较

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发表时间:
2003
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通讯作者:
H. Brückner
H. Brückner
中科院分区:
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文献类型:
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作者:
Ayşe Deniz Çardak;A. Yetişmeyen;H. Brückner

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本研究采用气相色谱/质谱联用技术(GC/MS)测定了驼奶、羊奶和牛奶中的游离脂肪酸(FFA)含量。牛奶样品在加入乙醇和2.5 M h2so4后,用乙醚/正庚烷混合物(1:1 v/v)提取。用氨基丙基柱分离游离脂肪酸。驼奶的总游离脂肪酸含量为13.64 μmol/10 ml,羊奶和牛奶的总游离脂肪酸含量分别为13.53 μmol/10 ml和22.56 μmol/10 ml。发现骆驼和山羊乳脂中含有c4 - c20的游离脂肪酸,而奶牛乳脂中不含c18: 2和c18: 3的游离脂肪酸。结果表明,骆驼奶中游离脂肪酸(c14)含量分别是羊奶和牛奶的2.8倍和1.9倍。
In this study, free fatty acids (FFA) contents of camel, goat and cow milk were determined by using Gas Chromatography/Mass Spectrometry (GC/MS). Milk samples were extracted by diethyl ether/n-heptane mixture (1:1 v/v) after addition of ethanol and 2.5 M H 2 SO 4 . The FFA isolation was carried out with an aminopropyl column. The total FFA concentration of camel milk was 13.64 μmol/10 ml milk and goat and cow milk contained 13.53 and 22.56 μmol/10 ml milk FFA, respectively. It was found that camel and goat milk fat had FFA with C 4 -C 2 0 , while cow milk fat contained no FFA with C 1 8 : 2 and C 1 8 : 3 . It was established that FFA contents ( C 1 4 ) concentration in camel milk was approximately 2.8-fold and 1.9-fold higher than that of goat and cow milk, respectively.