The COP9 signalosome interacts physically with SCFCOI1 and modulates jasmonate responses
The COP9 signalosome interacts physically with SCFCOI1 and modulates jasmonate responses
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DOI：
10.1105/tpc.010207
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发表时间：
2003-05-01
期刊：
影响因子：
11.6
通讯作者：
Deng, XW
中科院分区：
文献类型：
--
作者：
Feng, SH;Ma, LG;Deng, XW
COP9信号体(CSN)是一种进化上保守的富含细胞核的多蛋白复合体。CSN可能通过调节泛素-蛋白酶体介导的蛋白质降解，在光形态发生、生长素反应和花器官形成中发挥作用。COI1编码一个F-box蛋白，它是SCFCOI1 E3泛素连接酶的一个亚基，是茉莉酸(JA)反应所必需的。在这里，我们使用免疫共沉淀和凝胶过滤分析证明，内源性和表位标记的COI1形成SCFCOI1，并在体内与CSN直接相关。与coi1-1突变体一样，CSN功能降低的植株表现出对JA不敏感的根伸长表型，并且缺乏JA诱导的特异基因表达。基因组表达谱分析表明，JA触发的基因组表达严重依赖于COI1的剂量。更重要的是，大多数依赖COI1的茉莉酸应答基因也需要CSN功能，而且CSN丰度对茉莉酸应答很重要。此外，我们还发现COI1和CSN在调节大多数细胞通路中对JA反应的基因表达方面都是必不可少的。因此，CSN和SCFCOI1共同控制基因组表达，促进JA反应。
The COP9 signalosome (CSN) is an evolutionarily conserved, nucleus-enriched multiprotein complex. CSN plays roles in photomorphogenesis, auxin response, and floral organ formation, possibly via the regulation of ubiquitin-proteasome-mediated protein degradation. COI1 encodes an F-box protein, which is a subunit of SCFCOI1 E3 ubiquitin ligase, and is required for jasmonate (JA) responses. Here, we demonstrate using coimmunoprecipitation and gel-filtration analyses that endogenous as well as epitope-tagged COI1 forms SCFCOI1 and associates directly with CSN in vivo. Like the coi1-1 mutant, CSN reduction-of-function plants exhibited a JA-insensitive root elongation phenotype and an absence of JA-induced-specific gene expression. Genome expression profile analyses indicated that JA-triggered genome expression is critically dependent on COI1 dosage. More importantly, most of the COI1-dependent JA-responsive genes also required CSN function, and CSN abundance was shown to be important for JA responses. Furthermore, we showed that both COI1 and CSN are essential for modulating the expression of genes in most cellular pathways responsive to JA. Thus, CSN and SCFCOI1 work together to control genome expression and promote JA responses.