Biodegradation of Single-Walled Carbon Nanotubes in Macrophages through Respiratory Burst Modulation.
Biodegradation of Single-Walled Carbon Nanotubes in Macrophages through Respiratory Burst Modulation.
复制标题
通过呼吸爆发调节单壁碳纳米管在巨噬细胞中的生物降解
DOI：
10.3390/ijms17030409
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发表时间：
2016-03-22
影响因子：
5.6
通讯作者：
Liu JF
中科院分区：
文献类型：
--
作者：
Hou J;Wan B;Yang Y;Ren XM;Guo LH;Liu JF
碳纳米管（CNTs）的生物降解可能是暴露个体的毒性结果的主要决定因素之一。在这项研究中，我们采用了巨噬细胞/单核细胞模型，Raw 264. 7，研究呼吸爆发调制调节三种类型的单壁碳纳米管（SWCNTs）（原始，ox-和OH-SWCNTs）的生物降解的可行性。构建了模拟呼吸爆发酶促反应的人工流体，以揭示呼吸爆发在单壁碳纳米管生物降解中的作用。利用拉曼光谱、紫外-可见-近红外光谱和透射电镜对单壁碳纳米管的生物降解性进行了表征。我们的研究结果表明，ox-SWCNTs和OH-SWCNTs在佛波醇肉豆蔻酸酯乙酸酯（PMA）激活的巨噬细胞中的生物降解显着加速，这可以通过N-乙酰基-L-半胱氨酸（NAC）来阻止，而p-SWCNTs对生物降解具有抗性。在体外酶促体系中也观察到了类似的降解趋势，其降解速率顺序为OH-SWCNTs > ox-SWCNTs >> p-SWCNTs，表明呼吸爆发在加速SWCNTs生物降解中起着关键作用，SWCNTs上的缺陷位点可能是发生生物降解的先决条件。我们的研究结果可能会提供宝贵的线索，干预措施的发展，以减轻单壁碳纳米管的副作用，并将有助于设计更安全的单壁碳纳米管产品具有更高的生物降解性和更低的毒性。
The biodegradation of carbon nanotubes (CNTs) may be one of major determinants of the toxic outcomes in exposed individuals. In this study, we employed a macrophage/monocyte model, Raw264.7, to investigate the feasibility of regulating the biodegradation of three types of single-walled carbon nanotubes (SWCNTs) (pristine, ox-, and OH-SWCNTs) by respiratory burst modulation. An artificial fluid mimicking the enzymatic reactions of respiratory burst was constituted to reveal the role of respiratory burst played in SWCNT biodegradation. The biodegradation of SWCNTs were characterized by Raman, ultraviolet-visible-near-infrared spectroscopy, and transmission electron microscopy. Our results showed significantly accelerated biodegradation of ox-SWCNTs and OH-SWCNTs in macrophages activated by phorbol myristate acetate (PMA), which could be prevented by N-acetyl-l-cysteine (NAC), whereas p-SWCNTs were resistant to biodegradation. Similar tendencies were observed by using the in vitro enzymatic system, and the degradation rates of these SWCNTs are in the order of OH-SWCNTs > ox-SWCNTs >> p-SWCNTs, suggesting a pivotal role of respiratory burst in accelerating the biodegradation of SWCNTs and that defect sites on SWCNTs might be a prerequisite for the biodegradation to occur. Our findings might provide invaluable clues on the development of intervention measurements for relieving the side effects of SWCNTs and would help to design safer SWCNT products with higher biodegradability and less toxicity.