Differential gene expression between sensory neocortical areas:: Potential roles for Ten_m3 and Bcl6 in patterning visual and somatosensory pathways
Differential gene expression between sensory neocortical areas:: Potential roles for Ten_m3 and Bcl6 in patterning visual and somatosensory pathways
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DOI：
10.1093/cercor/bhm031
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发表时间：
2008-01-01
期刊：
影响因子：
3.7
通讯作者：
Sur, Mriganka
中科院分区：
文献类型：
--
作者：
Leamey, Catherine A.;Glendining, Kelly A.;Sur, Mriganka
成年人的新皮质区的特点是显着差异的细胞结构和连接，其功能的作用。这些差异的分子决定因素在很大程度上是未知的。我们进行了微阵列分析，以确定在传入和传出投射形成期间定义体感和视觉区域的分子。我们确定了122个分子的区域之间的差异表达，并确认了定量聚合酶链反应95%的20个基因测试。选择两个基因用于进一步研究：Bcl 6和Ten_m3。Bcl 6在出生后第0天在体感皮质第IV层的皮层浅层有高表达（P <0.05）。这已经减少了P3，但强烈的表达被发现在第V层锥体细胞的P7，并保持到成年。逆行追踪显示Bcl 6在皮质脊髓神经元表达。Ten_m3在尾侧皮质V层内以分级模式表达，与视觉皮质对应良好。逆行追踪和免疫组化显示Ten_m3在发育中的视路投射神经元的轴突束上沿着高度表达。过表达表明Ten_m3在体内促进嗜同性粘附和神经突生长。这表明Ten_m3在视觉通路的发育中起重要作用。
Adult neocortical areas are characterized by marked differences in cytoarchitecture and connectivity that underlie their functional roles. The molecular determinants of these differences are largely unknown. We performed a microarray analysis to identify molecules that define the somatosensory and visual areas during the time when afferent and efferent projections are forming. We identified 122 molecules that are differentially expressed between the regions and confirmed by quantitative polymerase chain reaction 95% of the 20 genes tested. Two genes were chosen for further investigation: Bcl6 and Ten_m3. Bcl6 was highly expressed in the superficial cortical plate corresponding to developing layer IV of somatosensory cortex at postnatal day (P) 0. This had diminished by P3, but strong expression was found in layer V pyramidal cells by P7 and was maintained until adulthood. Retrograde tracing showed that Bcl6 is expressed in corticospinal neurons. Ten_m3 was expressed in a graded pattern within layer V of caudal cortex that corresponds well with visual cortex. Retrograde tracing and immunostaining showed that Ten_m3 is highly expressed along axonal tracts of projection neurons of the developing visual pathway. Overexpression demonstrated that Ten_m3 promotes homophilic adhesion and neurite outgrowth in vivo. This suggests an important role for Ten_m3 in the development of the visual pathway.