Development of early porcine embryos in vitro and in vivo
Development of early porcine embryos in vitro and in vivo
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DOI：
10.1095/biolreprod59.2.451
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发表时间：
1998-08-01
影响因子：
3.6
通讯作者：
Prather, RS
中科院分区：
文献类型：
--
作者：
Macháty, Z;Day, BN;Prather, RS
对不同培养条件下早期猪胚胎的体外发育进行了评估，并与体内发育进行了比较。首先，收集单细胞和双细胞胚胎，并在 5% CO2 空气中分别在 20 微升液滴中培养 4 天。来自一侧输卵管的胚胎在 NCSU-23 中培养，来自对侧输卵管的胚胎在 KSOM/AA 中培养。与 KSOM/AA 中发育的胚胎相比，在 NCSU-23 中发育的胚胎具有更高的平均内细胞团 (ICM) 核数 (p = 0.025)。它们还具有较高的滋养外胚层 (TE) 和总核数 (p = 0.001)，而 ICM 与 TE 核的平均比率没有差异 (p = 0.731)。当测试不同气体气氛的影响时，在空气中含有 5% CO 的气氛中培养的胚胎的 TE 和总核数（分别为 p < 0.01 和 p < 0.025）高于在 5% CO2:5 % O-2:90% N-2 下发育的胚胎。接下来，将 NCSU-23 中培养的胚胎的发育与体内培养的胚胎的发育进行比较。 4天孵化结束时，培养胚胎比体内发育的胚胎具有更高的核数量和更高的ICM与TE核比例（p < 0.001）。最后，将在NCSU-23或体内发育的胚胎移植到受体体内。到妊娠第 40 天，37.1 +/- 15.3% 的体外孵化胚胎和 53.8 +/- 15.3% 的体内孵化胚胎形成受孕。这些结果表明，尽管体外条件导致核数量较低，但培养的胚胎具有发育能力。
In vitro development of early porcine embryos under different culture conditions was evaluated and compared to in vivo development. First, one- and two-cell embryos were collected and cultured individually in 20-mu l drops under 5% CO2 in air for 4 days. Embryos from one oviduct were cultured in NCSU-23, and those from the contralateral oviduct were cultured in KSOM/AA. The embryos developed in NCSU-23 had a higher mean number of inner cell mass (ICM) nuclei compared to those developed in KSOM/AA (p = 0.025). They also had higher trophectoderm (TE) and total nuclear number (p = 0.001), while there was no difference in the average ratio of ICM to TE nuclei (p = 0.731). When the effect of different gas atmospheres was tested, the numbers of TE and total nuclei were higher (p < 0.01 and p < 0.025, respectively) in embryos cultured in an atmosphere with 5% CO, in air than in those developed under 5% CO2:5 % O-2:90% N-2. Next the development of embryos cultured in NCSU-23 was compared to that of embryos incubated in vivo. By the end of the 4-day incubation, the cultured embryos had higher nuclear numbers and a higher ratio of ICM to TE nuclei than those developed in vivo (p < 0.001).Finally, the embryos that developed in NCSU-23 or in vivo were transferred into recipients. By Day 40 of pregnancy, 37.1 +/- 15.3% of the in vitro- and 53.8 +/- 15.3% of the in vivo-incubated embryos formed conceptuses. These results indicate that despite the lower nuclear numbers caused by in vitro conditions, the cultured embryos were developmentally competent.