Gene expression of sphingolipid metabolism pathways is altered in hidradenitis suppurativa
Gene expression of sphingolipid metabolism pathways is altered in hidradenitis suppurativa
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DOI：
10.1016/j.jaad.2017.03.016
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发表时间：
2017-08-01
影响因子：
13.8
通讯作者：
Elston, Dirk
中科院分区：
文献类型：
--
作者：
Dany, Mohammed;Elston, Dirk
背景：化脓性汗腺炎（HS）是一种使人衰弱的皮肤病，其特征是慢性炎症引起的疼痛性复发性结节和脓肿。据信 HS 发展的早期事件发生在毛囊毛囊皮脂腺单位；然而，这种机制背后的信号通路尚不清楚。神经酰胺等鞘脂是皮肤和附属器的重要组成部分，具有重要的结构和信号作用。目的：我们试图探讨热射病中鞘脂代谢途径相关酶的基因表达是否发生改变。方法：使用包含30个样本的微阵列数据集来比较热射病患者（n = 17）炎症性皮损中鞘脂相关酶的表达与临床健康皮肤组织中的表达（n = 13）。使用Gene Expression Omnibus 2R分析鞘脂代谢相关基因的差异表达。结果：HS病变皮肤样本中生成神经酰胺和鞘磷脂的酶的表达显着降低，将神经酰胺分解代谢为鞘氨醇的酶的表达增加，并将神经酰胺转化为半乳糖神经酰胺和神经节苷脂的酶的表达增加。局限性：本研究的局限性包括评估鞘脂相关酶的表达，但未评估相关鞘脂的水平。结论：我们的研究表明，与正常皮肤相比，HS 病变皮肤的鞘脂代谢发生了改变。
Background: Hidradenitis suppurativa (HS) is a debilitating skin disease characterized by painful recurrent nodules and abscesses caused by chronic inflammation. Early events in the development of HS are believed to occur in the folliculopilosebaceous unit; however, the signaling pathways behind this mechanism are unknown. Sphingolipids, such as ceramide, are essential components of the skin and appendages and have important structural and signaling roles.Objective: We sought to explore whether the gene expression of enzymes involved in sphingolipid metabolic pathways is altered in HS.Methods: A microarray data set including 30 samples was used to compare the expression of sphingolipid-related enzymes in inflammatory skin lesions from HS patients (n = 17) with the expression in clinically healthy skin tissue (n = 13). Differential expression of sphingolipid metabolismerelated genes was analyzed using Gene Expression Omnibus 2R.Results: HS lesional skin samples have significantly decreased expression of enzymes generating ceramide and sphingomyelin, increased expression of enzymes catabolizing ceramide to sphingosine, and increased expression of enzymes converting ceramide to galactosylceramide and gangliosides. Limitations: Limitations of this study include assessing the expression of sphingolipid-related enzymes without assessing the levels of the related sphingolipids.Conclusion: Our study suggests that sphingolipid metabolism is altered in HS lesional skin compared with normal skin.