Supplementary References
Supplementary References
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通讯作者：
Davis Ge-;Martin Bm-;Fiala Jc-;Kirov Sa-;Feinberg Md-;Petrak Lj-;George P-;Goddard Ca-;Harris Km-
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作者：
Davis Ge-;Martin Bm-;Fiala Jc-;Kirov Sa-;Feinberg Md-;Petrak Lj-;George P-;Goddard Ca-;Harris Km-
Bies-Etheve et al.，补充方法序列分析序列比对使用BLACKS或PSI-BLAST程序进行（Altschul等，1997）。多重比对和系统发育分析使用CLUSTALW，默认参数如（Larkin et al，2007）中所述。突变体基因分型为了确认spt 5l-1突变体中T-DNA的存在，用LBA 1（T-DNA特异性）和617引物扩增基因组DNA（所用的所有引物在补充信息表1中描述）。为了跟踪内源性SPT 5LIKE拷贝的存在，我们使用引物616和617。在spt 5l-2突变体中，使用LB 3和616引物测试T-DNA插入，并且使用引物643和670测试内源基因。（1997）Gapped BLAST and PSI-BLAST：a new generation of protein database search programs.
Supplementary informations Bies-Etheve et al., Supplementary Methods Sequence analysis Sequence alignments were carried out using either the BLASTN or PSI-BLAST programmes (Altschul et al, 1997). Multiple alignment and phylogenetic analyses were with CLUSTALW with default parameters as described in (Larkin et al, 2007). Mutant genotyping To confirm the presence of T-DNA in spt5l-1 mutant, genomic DNA was amplified with LBA1 (T-DNA specific) and 617 primers (all primers used are described in supplementary information Table1). To follow the presence of the endogenous SPT5LIKE copy we used primers 616 and 617. In spt5l-2 mutant T-DNA insertion was tested using LB3 and 616 primers and the endogenous gene with primers 643 and 670. (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.