Immunoelectron microscopic localization of actin in neurites of cultured embryonic chick dorsal root ganglia: actin is a component of granular, microtubule-associated crossbridges.
Immunoelectron microscopic localization of actin in neurites of cultured embryonic chick dorsal root ganglia: actin is a component of granular, microtubule-associated crossbridges.
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培养胚胎鸡背根神经节神经突中肌动蛋白的免疫电子显微镜定位：肌动蛋白是颗粒状微管相关横桥的组成部分。
DOI：
10.1016/0006-8993(88)90441-6
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发表时间：
1988
期刊：
影响因子：
2.9
通讯作者：
Lee,H
中科院分区：
文献类型：
--
作者：
Nagele,RG;Kosciuk,MC;Hunter,ET;Bush,KT;Lee,H
轴突已被证明含有大量沿其长度分布的肌动蛋白。然而，由于普遍缺乏关于轴突中这种蛋白质的结构和组织状态的信息，因此很难将其分配给功能角色。在本研究中，我们用免疫金标记法对培养的鸡胚胎背根神经节的神经突起进行了研究：(1)确定了肌动蛋白在神经突起中的分布；(2)鉴定了含有肌动蛋白的结构；(3)揭示了肌动蛋白是否优先与微管相关。结果表明，肌动蛋白存在于整个神经突起中，但主要组织成短丝，这些短丝几乎只定位于颗粒状微管相关的交叉桥。我们认为这些短的肌动蛋白细丝是慢组分b多肽的超分子‘载体复合体’框架的一部分。此外，含有肌动蛋白的交叉桥通常与膜结合细胞器的表面密切相关。这表明肌动蛋白和微管相关的交叉桥在某种程度上参与了快速轴突运输，尽管它们参与这一过程的性质仍然需要解决。
Axons have been shown to contain substantial quantities of actin distributed along their length. However, the general lack of information on the structure and organizational state of this protein in axons has made it difficult to assign it a functional role. In the present study, we used electron microscopic immunocytochemistry (immunogold labeling) on neurites from cultured embryonic chick dorsal root ganglia to: (1) determine the distribution of actin in neurites: (2) identify actin-containing structures; and (3) reveal whether or not actin is associated preferentially with microtubules. Results show that actin is present throughout neurites but is organized primarily into short filaments that are localized almost exclusively to granular, microtubule-associated crossbridges. We propose that these short actin filaments are part of the framework of a supramolecular ‘carrier complex’ for the slow component b polypeptides. In addition, actin-containing crossbridges are often closely associated with the surfaces of membrane-bound organelles. This suggests that actin and microtubule-associated crossbridges are involved somehow in fast axonal transport, although the nature of their participation in this process still needs to be resolved.