HUMAN COLLAGEN GENES ENCODING BASEMENT-MEMBRANE ALPHA-1(IV) AND ALPHA-2(IV) CHAINS MAP TO THE DISTAL LONG ARM OF CHROMOSOME-13
HUMAN COLLAGEN GENES ENCODING BASEMENT-MEMBRANE ALPHA-1(IV) AND ALPHA-2(IV) CHAINS MAP TO THE DISTAL LONG ARM OF CHROMOSOME-13
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DOI：
10.1073/pnas.84.2.512
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发表时间：
1987-01-01
影响因子：
11.1
通讯作者：
MYERS, JC
中科院分区：
文献类型：
--
作者：
GRIFFIN, CA;EMANUEL, BS;MYERS, JC
至少20个基因编码结构相关的胶原蛋白链，其包含> 10种同源或异源三聚体类型。这个多基因家族的六个成员被分配到人类基因组的五条染色体上。两个I型基因α 1和α 2分别位于染色体17和7上，α 1（II）基因位于染色体12上。我们最近将α 1（III）和α 2（V）基因定位到q24.3 →。2号染色体q31区域提供了胶原基因不完全分散的唯一证据。为了进一步确定它们的组织，我们和其他人将α 1（IV）基因定位于染色体13，并在我们的实验中通过原位杂交将该基因亚定位于带q34。在此，我们通过将人α 2（IV）cDNA克隆与啮齿动物-人杂交体和中期染色体杂交，证明了α 2 IV型基因座也存在于染色体13的远端长臂上。据我们所知，这些研究代表了编码相同胶原类型的两条多肽链的基因之间的联系的唯一证明。
At least 20 genes encode the structurally related collagen chains that comprise > 10 homo- or heterotrimeric types. Six members of this multigene family have been assigned to five chromosomes in the human genome. The two type I genes, .alpha.1, and .alpha.2, are located on chromosome 17 and 7, respectively, and the .alpha.1(II) gene is located on chromosome 12. Our recent mapping of the .alpha.1(III) and .alpha.2(V) genes to the q24.3 .fwdarw. q31 region of chromosomes 2 provided the only evidence that the collagen genes are not entirely dispersed. To further determine their organization, we and others localized the .alpha.1(IV) gene to chromosome 13 and in our experiments sublocalized the gene to band q34 by in situ hybridization. Here we show the presence of the .alpha.2 type IV locus also on the distal long arm of chromosome 13 by hybridizing a human .alpha.2(IV) cDNA clone to rodent-human hybrids and to metaphase chromosomes. To our knowledge, these studies represent the only demonstration of linkage between genes encoding both polypeptide chains of the same collagen type.