RNA editing is abundant and correlates with task performance in a social bumblebee
RNA editing is abundant and correlates with task performance in a social bumblebee
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DOI：
10.1038/s41467-019-09543-w
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发表时间：
2019-04-08
影响因子：
16.6
通讯作者：
Bloch, Guy
中科院分区：
文献类型：
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作者：
Porath, Hagit T.;Hazan, Esther;Bloch, Guy
大黄蜂Bombus terrestris的群体在遗传相似的全姐妹工蜂中具有广泛的表型变异，这表明表观遗传过程具有重要作用。在这里，我们报告了在大黄蜂中高水平的ADAR介导的RNA编辑，尽管缺乏ADAR1同源基因。我们确定了115万个独特的基因组位点，以及100个蛋白质编码基因中的164个重新编码位点，包括离子通道、转运体和受体，这些基因预计会影响大脑的功能和行为。在其他昆虫、头足类甚至哺乳动物中，一些编辑过的站点也进行了类似的编辑。蛋白质编码和非编码转录本的全局编辑水平与任务表现(育儿与觅食)弱相关，但不受优势等级或已知影响生理和行为的保幼激素的影响。综上所述，我们的发现表明，自然行为的蜜蜂的大脑编辑水平很高，可能受到与育儿或觅食活动相关的相对短期影响的调节。
Colonies of the bumblebee Bombus terrestris are characterized by wide phenotypic variability among genetically similar full-sister workers, suggesting a major role for epigenetic processes. Here, we report a high level of ADAR-mediated RNA editing in the bumblebee, despite the lack of an ADAR1-homolog. We identify 1.15 million unique genomic sites, and 164 recoding sites residing in 100 protein coding genes, including ion channels, transporters, and receptors predicted to affect brain function and behavior. Some edited sites are similarly edited in other insects, cephalopods and even mammals. The global editing level of protein coding and non-coding transcripts weakly correlates with task performance (brood care vs. foraging), but not affected by dominance rank or juvenile hormone known to influence physiology and behavior. Taken together, our findings show that brain editing levels are high in naturally behaving bees, and may be regulated by relatively short-term effects associated with brood care or foraging activities.