THE EFFECT OF DIFFERENT FIXATIVES ON CHROMATIN - CYTOCHEMICAL AND ULTRASTRUCTURAL APPROACHES
THE EFFECT OF DIFFERENT FIXATIVES ON CHROMATIN - CYTOCHEMICAL AND ULTRASTRUCTURAL APPROACHES
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DOI：
10.1007/bf01003288
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发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
ROMANINI, MGM
中科院分区：
文献类型：
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作者：
FRASCHINI, A;PELLICCIARI, C;ROMANINI, MGM
本研究探讨了两种固定剂对染色质的影响。第一类（丙烯醛、戊二醛）产生高度的超微结构保存。另一类是广泛用于细胞化学和细胞遗传学的固定剂（乙酸、体积比3：1的甲醇-乙酸、单独的甲醇、甲醛）。制备体外固定的成年大鼠淋巴细胞，用于涂片的EM或显微密度测定评价。对它们的总蛋白、核碱性蛋白和DNA含量的变化进行了评估。DNA被确定为Feulgen阳性物质，并通过其与嵌入染料（甲基绿色，对双链多核苷酸具有特异性）的结合来确定。一些固定剂通过作用于染色质纤维的特定组分来破坏染色质组织。它们可以被认为是原地破坏剂。对某些醛类固定剂的超微结构和细胞化学研究提出了重新评价。
This study explores the effects of 2 types of fixative on chromatin. The 1st type (acrolein, glutaraldehyde) engenders a high degree of ultrastructural preservation. The other type are fixatives that are widely used in cytochemistry and cytogenetics (acetic acid, 3:1 by vol. methanol-acetic acid, methanol alone, formaldehyde). Lymphocytes of adult rats so-fixed in vitro were prepared for EM or microdensitometric evaluations of smears. Assessments were made of variations in their total protein, nuclear basic protein and DNA contents. DNA was determined both as Feulgen-positive material and by its binding of intercalating dyes (Methyl Green, specific for double-stranded polynucleotides). Some fixatives break up the chromatin organization by acting on particular components of chromatin fibers. They can be considered destructive agents in situ. A reevaluation of some aldehyde fixatives is proposed for both ultrastructural and cytochemical research.