A novel biocatalyst for efficient production of 2-oxo-carboxylates using glycerol as the cost-effective carbon source.
A novel biocatalyst for efficient production of 2-oxo-carboxylates using glycerol as the cost-effective carbon source.
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一种使用甘油作为具有成本效益的碳源高效生产 2-氧代羧酸盐的新型生物催化剂
DOI：
10.1186/s13068-015-0368-y
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发表时间：
2015
影响因子：
6.3
通讯作者：
Ma C
中科院分区：
文献类型：
--
作者：
Wang Y;Zhang Y;Jiang T;Meng J;Sheng B;Yang C;Gao C;Xu P;Ma C
作为生物燃料生产过程中的主要副产品，甘油的过剩量显着增加。探索甘油利用的替代途径对于生物燃料的可持续性非常重要。开发了一种新型生物催化剂，可以用甘油制备用于生产 2-氧代-羧酸盐。首先，通过删除 lldR 来重建恶臭假单胞菌 KT2440，以开发组成型表达不依赖于 NAD 的乳酸脱氢酶 (iLDH) 的突变体。然后，异源表达玻璃颤菌血红蛋白（VHb）以进一步提高生物转化活性。重建菌株P. putida KT2440 (ΔlldR)/pBSPPcGm-vgb在以甘油作为碳源培养时表现出高iLDH活性。这种经济高效的生物催化剂可以有效地从 dl-乳酸和 dl-2-羟基丁酸生产丙酮酸和 2-氧代丁酸，摩尔转化率分别高达 91.9% 和 99.8%。该工艺不仅是生产 2-氧代羧酸盐的一种有前景的替代方案，而且也是制备用于甘油增值利用的高效生物催化剂的范例。
The surplus of glycerol has increased remarkably as a main byproduct during the biofuel’s production. Exploiting an alternative route for glycerol utilization is significantly important for sustainability of biofuels. A novel biocatalyst that could be prepared from glycerol for producing 2-oxo-carboxylates was developed. First, Pseudomonas putida KT2440 was reconstructed by deleting lldR to develop a mutant expressing the NAD-independent lactate dehydrogenases (iLDHs) constitutively. Then, the Vitreoscilla hemoglobin (VHb) was heterologously expressed to further improve the biotransformation activity. The reconstructed strain, P. putida KT2440 (ΔlldR)/pBSPPcGm-vgb, exhibited high activities of iLDHs when cultured with glycerol as the carbon source. This cost-effective biocatalyst could efficiently produce pyruvate and 2-oxobutyrate from dl-lactate and dl-2-hydroxybutyrate with high molar conversion rates of 91.9 and 99.8 %, respectively. The process would not only be a promising alternative for the production of 2-oxo-carboxylates, but also be an example for preparation of efficient biocatalysts for the value-added utilization of glycerol.