Monitoring contractility in cardiac tissue with cellular resolution using biointegrated microlasers
Monitoring contractility in cardiac tissue with cellular resolution using biointegrated microlasers
复制标题
DOI：
10.1038/s41566-020-0631-z
复制
发表时间：
2020-06-15
期刊：
影响因子：
35
通讯作者：
Gather, Malte C.
中科院分区：
文献类型：
--
作者：
Schubert, Marcel;Woolfson, Lewis;Gather, Malte C.
将微盘激光器结合到心脏细胞中，可以实现细胞分辨率的心脏收缩的全光学记录。心脏细胞的收缩性是描述心脏跳动的生物力学特性的关键参数，但单细胞分辨率的三维心脏组织的功能监测仍然是一个重大挑战。在这里，我们引入微观回音壁模式激光到心脏细胞，实现全光学记录的瞬态心脏收缩轮廓与细胞分辨率。微激光器对折射率局部变化的明亮发射和高光谱灵敏度使其能够长期跟踪单个心脏细胞，监测药物给药，精确测量活斑马鱼器官尺度的收缩力，并通过数百微米的大鼠心脏组织进行强大的收缩感测。我们的研究揭示了肌节蛋白密度的变化是心脏收缩的一个潜在因素。更广泛地说，使用新型的微米和纳米级激光器作为非侵入性的生物集成光学传感器带来了新的机会，以细胞分辨率监测各种生理参数。
The incorporation of microdisk lasers into heart cells allows all-optical recording of cardiac contraction with cellular resolution.The contractility of cardiac cells is a key parameter that describes the biomechanical characteristics of the beating heart, but functional monitoring of three-dimensional cardiac tissue with single-cell resolution remains a major challenge. Here, we introduce microscopic whispering-gallery-mode lasers into cardiac cells to realize all-optical recording of transient cardiac contraction profiles with cellular resolution. The brilliant emission and high spectral sensitivity of microlasers to local changes in refractive index enable long-term tracking of individual cardiac cells, monitoring of drug administration, accurate measurements of organ-scale contractility in live zebrafish, and robust contractility sensing through hundreds of micrometres of rat heart tissue. Our study reveals changes in sarcomeric protein density as an underlying factor to cardiac contraction. More broadly, the use of novel micro- and nanoscopic lasers as non-invasive, biointegrated optical sensors brings new opportunities to monitor a wide range of physiological parameters with cellular resolution.