Typical cutaneous small‐vessel vasculitis induced by combined injection of antiphosphatidylserine/prothrombin complex antibody and anti‐LAMP‐2 antibody in normal rats
Typical cutaneous small‐vessel vasculitis induced by combined injection of antiphosphatidylserine/prothrombin complex antibody and anti‐LAMP‐2 antibody in normal rats
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DOI：
10.1111/1346-8138.16523
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发表时间：
2022-07
期刊：
影响因子：
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通讯作者：
T. Kawakami;I. Nakade;Y. Tamura;Fuyu Ito;Y. Nishibata;S. Masuda;U. Tomaru;A. Ishizu
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文献类型：
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作者：
T. Kawakami;I. Nakade;Y. Tamura;Fuyu Ito;Y. Nishibata;S. Masuda;U. Tomaru;A. Ishizu
我们之前报道过，IgA 血管炎和皮肤动脉炎可能与抗磷脂酰丝氨酸/凝血酶原复合物（抗 PS/PT）抗体和溶酶体相关膜蛋白 2（LAMP-2）的存在相关。皮下注射组蛋白后静脉注射抗PS/PT抗体诱发皮肤血管炎大鼠皮肤组织样本中LAMP-2 mRNA拷贝数显着高于无皮肤血管炎大鼠。我们发现，在组蛋白皮肤引发后静脉注射抗 PS/PT 抗体诱导的皮肤血管炎大鼠中，受影响血管的中性粒细胞和血管内皮细胞中 LAMP-2 蛋白过度表达。我们在组蛋白皮肤引发后静脉注射抗 PS/PT 抗体和抗 LAMP-2 抗体的大鼠皮肤中发现了典型的皮肤小血管炎。我们认为，在血清中引入皮肤局部组蛋白和抗 PS/PT 抗体可以将 LAMP-2 移动到中性粒细胞和血管内皮细胞的细胞表面，而抗 LAMP-2 抗体可以通过典型皮肤小血管炎中的抗原特异性结合来桥接这些细胞。
We previously reported that IgA vasculitis and cutaneous arteritis could be dependently associated with the presence of the antiphosphatidylserine/prothrombin complex (anti‐PS/PT) antibody and lysosomal‐associated membrane protein‐2 (LAMP‐2). The copy number of LAMP‐2 mRNA in skin tissue samples from rats with cutaneous vasculitis induced by intravenous administration of anti‐PS/PT antibody after subcutaneous histone injection was significantly higher than in those without cutaneous vasculitis. We found LAMP‐2 protein overexpression in neutrophils and vascular endothelial cells of the affected blood vessels in rats with cutaneous vasculitis induced by intravenous administration of anti‐PS/PT antibody after cutaneous priming by histones. We found typical cutaneous small‐vessel vasculitis in the skin of rats given intravenous injection of both anti‐PS/PT antibody and anti‐LAMP‐2 antibody after cutaneous priming by histones. We suggested that the introduction of skin local histones and anti‐PS/PT antibody in serum could move LAMP‐2 to the cell surface of neutrophils and vascular endothelial cells, and that anti‐LAMP‐2 antibody could bridge these cells through antigen‐specific binding in typical cutaneous small‐vessel vasculitis.