Molecular cloning of a glibenclamide-sensitive, voltage-gated potassium channel expressed in rabbit kidney.
Molecular cloning of a glibenclamide-sensitive, voltage-gated potassium channel expressed in rabbit kidney.
复制标题
兔肾中表达的格列本脲敏感、电压门控钾通道的分子克隆。
DOI：
10.1172/jci118700
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Desir,GV
中科院分区：
文献类型：
--
作者：
Yao,X;Chang,AY;Boulpaep,EL;Segal,AS;Desir,GV
Shaker基因编码电压门控钾通道（Kv）。我们以前已经表明，来自Shaker亚家族Kv1.1，1.2，1.4的基因在兔肾中表达。最近的功能和分子证据表明，肾髓质细胞系GRB-PAP 1的主要钾传导由Shaker样钾通道组成。我们现在报告的分子克隆和功能表达的一个新的振动相关的电压门控钾通道，rabKv1.3，这是在兔脑和肾髓质表达。该蛋白质，预测为513个氨基酸长，是最密切相关的Kv1.3家族，虽然它显着不同，从该家庭的其他成员在氨基末端。在非洲爪蟾卵母细胞中，rabKv1.3 cRNA表达的电压激活的K电流的动力学特征类似于Kv1.3家族的其他成员。然而，与先前描述的Shaker通道不同，它对格列本脲敏感，并且其单通道电导饱和。这是第一个报告的功能性表达的电压门控钾通道克隆在肾脏中表达。我们的结论是，rabKv1.3是一个新的Shaker超家族的成员，可能在肾钾转运中发挥重要作用。
Shaker genes encode voltage-gated potassium channels (Kv). We have shown previously that genes from Shaker subfamilies Kv1.1, 1.2, 1.4 are expressed in rabbit kidney. Recent functional and molecular evidence indicate that the predominant potassium conductance of the kidney medullary cell line GRB-PAP1 is composed of Shaker-like potassium channels. We now report the molecular cloning and functional expression of a new Shaker-related voltage-gated potassium channel, rabKv1.3, that is expressed in rabbit brain and kidney medulla. The protein, predicted to be 513 amino acids long, is most closely related to the Kv1.3 family although it differs significantly from other members of that family at the amino terminus. In Xenopus oocytes, rabKv1.3 cRNA expresses a voltage activated K current with kinetic characteristics similar to other members of the Kv1.3 family. However, unlike previously described Shaker channels, it is sensitive to glibenclamide and its single channel conductance saturates. This is the first report of the functional expression of a voltage-gated K channel clone expressed in kidney. We conclude that rabKv1.3 is a novel member of the Shaker superfamily that may play an important role in renal potassium transport.