A comparative study of induced pluripotent stem cells generated from frozen, stocked bone marrow‐ and adipose tissue‐derived mesenchymal stem cells
A comparative study of induced pluripotent stem cells generated from frozen, stocked bone marrow‐ and adipose tissue‐derived mesenchymal stem cells
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DOI：
10.1002/term.428
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发表时间：
2012-04
影响因子：
3.3
通讯作者：
Hiroe Ohnishi;Yasuaki Oda;T. Aoki;M. Tadokoro;Yoshihiro Katsube;H. Ohgushi;K. Hattori;S. Yuba
中科院分区：
文献类型：
--
作者：
Hiroe Ohnishi;Yasuaki Oda;T. Aoki;M. Tadokoro;Yoshihiro Katsube;H. Ohgushi;K. Hattori;S. Yuba
骨髓间充质干细胞(BMSCs)和脂肪组织间充质干细胞(AMSCs)已在临床上用于组织再生，但其增殖/分化潜能有限。最近，诱导多能干细胞(IPSCs)在再生医学领域获得了广泛的兴趣，它具有几乎无限的增殖和分化为所有三个生殖层细胞的潜力。在这里，我们从冻存的AMSCs和BMSCs中培养出IPSCs，并通过比较分析检测了它们的生物学特性。尽管从BMSCs分化为IPSC比从AMSCs诱导更具挑战性，但两个IPSC群体都表达多能标志物，如阶段特异性胚胎抗原(SSEA)-3、SSEA-4、肿瘤相关抗原(Tras)TRA-1-60和TRA-1-81、Oct3/4和NANOG。此外，在体外和体内，这两个细胞群体都很好地分化为三个生殖层来源的细胞。这些结果表明，冷冻的AMSCs/BMSCs来源的IPSCs表现出同样可接受的IPSC特性，并具有作为一种替代自体干细胞来源的临床应用潜力。版权所有©2011 John Wiley&Sons，Ltd.
Bone marrow‐derived mesenchymal stem cells (BMSCs) and adipose tissue‐derived mesenchymal stem cells (AMSCs) have been used clinically for tissue regeneration; however, their proliferation/differentiation potentials are limited. Recently, induced pluripotent stem cells (iPSCs), known to have nearly unlimited potential to proliferate and differentiate into cells of all three germ layers, have gained wide interest in regenerative medicine. Here, we generated iPSCs from frozen‐stocked AMSCs and BMSCs and examined their biological characteristics by comparative analyses. Although the iPSCs were more challenging to generate from the BMSCs than the AMSCs, both iPSC populations expressed pluripotent markers, such as stage‐specific embryonic antigen (SSEA)‐3, SSEA‐4, tumour‐related antigens (TRAs) TRA‐1‐60 and TRA‐1‐81, OCT3/4 and NANOG. Furthermore, both cell populations differentiated well into three germ layer‐derived cells, both in vitro and in vivo. These results indicate that iPSCs derived from frozen AMSCs/BMSCs exhibit equally acceptable iPSC characteristics and have potential in clinical applications as an alternative source of autogenous stem cells. Copyright © 2011 John Wiley & Sons, Ltd.