Melatonin reprogrammes proteomic profile in light-exposed retina in vivo.
Melatonin reprogrammes proteomic profile in light-exposed retina in vivo.
复制标题
褪黑素在体内重编程暴露于光的视网膜中的蛋白质组谱。
DOI：
10.1016/j.ijbiomac.2010.04.013
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发表时间：
2010
影响因子：
8.2
通讯作者：
Jahng,WanJin
中科院分区：
文献类型：
--
作者：
Zhang,Ruonan;Hrushesky,WilliamJM;Wood,PatriciaA;Lee,SungHaeng;Hunt,RichardC;Jahng,WanJin
褪黑素是一种由松果体和视网膜合成的有机小分子，具有多种生理功能，如生物钟起搏器和抗氧化剂。视网膜褪黑激素受光线下调，白天几乎检测不到。在长时间的光照下，视网膜中没有褪黑激素，这可能会导致光诱导的视网膜退化。我们试图用蛋白质组学的方法研究褪黑素在光暴露的视网膜中的影响。我们将小鼠暴露在光照(250-300lux)12小时，然后黑暗12小时，或者相同强度的连续光照7天。在一半暴露在持续光照下的动物中，每晚都会注射褪黑激素。对这三组动物视网膜的蛋白质组学分析表明，在持续光照下显著上调的五种蛋白质，在褪黑素处理后下调。这5个蛋白分别为波形蛋白、丝氨酸/苏氨酸蛋白磷酸酶2A、Rab GDP解离抑制因子α、鸟嘌呤核苷酸结合蛋白GoAla和视黄醛结合蛋白。已知这五种蛋白质参与了几个细胞过程，这些过程可能有助于光诱导的视网膜退化。本研究中褪黑素靶蛋白的鉴定为进一步研究褪黑素在预防或治疗光诱导的视网膜变性中的作用奠定了基础。
Melatonin, a small organic molecule synthesized by the pineal gland and the retina, has a variety of physiologic functions such as circadian clock pacemaker and antioxidant. Retinal melatonin is down-regulated by light and is barely detectable during the day. The absence of melatonin in the retina during prolonged light exposure may contribute to light-induced retinal degeneration. We sought to investigate the impact of melatonin in the light-exposed retina using proteomic approaches. We exposed mice to either light (250–300lux) for 12h followed by 12h of darkness or the same intensity of continuous light for 7 days. In half of the animals exposed to continuous light, melatonin was injected each night. Proteomic analysis of the retina from these three groups of animals showed that five proteins prominently up-regulated by constant light were down-regulated by melatonin treatment. These five proteins were identified as vimentin, serine/threonine-protein phosphatase 2A, Rab GDP dissociation inhibitor alpha, guanine nucleotide-binding protein Goalpha, and retinaldehyde-binding protein. These five proteins are known to be involved in several cellular processes that may contribute to light-induced retinal degeneration. Identification of melatonin target proteins in our study provides a basis for future studies on melatonin's potential in preventing or treating light-induced retinal degeneration.